Abstract: This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5?-CAAGCAGAAGACGGCATACGAGAT-N5 to 15-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3?, wherein N5 to 15 indicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Abstract: This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5?-CAAGCAGAAGACGGCATACGAGAT-N5 to 15-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3?, wherein N5 to 15 indicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Abstract: Prediction model area environment representative data that representatively indicates an environment of a prediction model area is generated based on prediction model area environmental data and prediction model area data. Additionally, prediction model area crop characteristic representative data that represents a characteristic of a crop cultivated in the prediction model area is generated. Based on the prediction model area environment representative data and the prediction model area crop characteristic representative data, a crop characteristic prediction model is generated.

Abstract: This invention provides a DNA probe that is applicable to a DNA library prepared in a simple manner with excellent reproducibility. Such DNA probe is produced by a method comprising steps of performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at a high concentration, so as to obtain a DNA fragments with the use of the genomic DNA as a template; determining the nucleotide sequence of the resulting DNA fragments; and, on the basis of the nucleotide sequence of the DNA fragments obtained in the step above, designing a DNA probe used for detecting a DNA fragment.

Abstract: When preparing a DNA library via a nucleic acid amplification reaction using a random primer in a convenient and highly reproducible manner, amplification of DNA fragments derived from the chloroplast genome is reduced to a significant extent. A random primer comprises oligonucleotides selected from oligonucleotides group represented by TAAGAGACAGNN excluding those in which 2 bases at the 3? terminus are TG and oligonucleotides group represented by TAAGAGACAGNNN excluding those in which 3 bases at the 3? terminus are TGC.