Patents by Inventor Minoru Ko

Minoru Ko has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220364058
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.
    Type: Application
    Filed: June 21, 2022
    Publication date: November 17, 2022
    Applicant: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Patent number: 11396644
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.
    Type: Grant
    Filed: January 31, 2017
    Date of Patent: July 26, 2022
    Assignee: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Publication number: 20210395692
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being stable and highly efficient. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by attenuating differentiation resistance of a pluripotent stem cell to generate a pluripotent stem cell that actively proceeds to a differentiated cell type has been found, and thus the present invention has been completed.
    Type: Application
    Filed: September 3, 2021
    Publication date: December 23, 2021
    Applicant: KEIO UNIVERSITY
    Inventors: Minoru Ko, Tomohiko Akiyama
  • Patent number: 11136552
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being stable and highly efficient. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by attenuating differentiation resistance of a pluripotent stem cell to generate a pluripotent stem cell that actively proceeds to a differentiated cell type has been found, and thus the present invention has been completed.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: October 5, 2021
    Assignee: KEIO UNIVERSITY
    Inventors: Minoru Ko, Tomohiko Akiyama
  • Publication number: 20210277347
    Abstract: A cell culture vessel includes: a base member having a plurality of recesses; and a frame member having through-holes and configured to be detachable from and attachable to the base member, wherein when the frame member is stacked on the base member, the plurality of recesses and the through-holes communicate with each other, the frame member includes a plurality of kinds of frame members, and the plurality of kinds of frame members have mutually different combinations of the through-holes communicating with the plurality of recesses.
    Type: Application
    Filed: March 2, 2021
    Publication date: September 9, 2021
    Inventors: Tomoaki NAKAYAMA, Shinnosuke KOSHIZUKA, Takeru SUZUKI, Ryuya MASHIKO, Minoru KO
  • Publication number: 20210277345
    Abstract: A cell-containing container includes at least one recessed part accommodating cells, in which the cells adhere to a bottom surface of the recessed part, and a cell density in a first region of the bottom surface is 250 to 20,000 cells/mm2, and a cell density in a second region surrounding a periphery of the first region is less than 250 cells/mm2.
    Type: Application
    Filed: March 3, 2021
    Publication date: September 9, 2021
    Inventors: Tomoyuki ARATANI, Daisuke TAKAGI, Hidekazu YAGINUMA, Waka LIN, Shinnosuke KOSHIZUKA, Ryuya MASHIKO, Tomoaki NAKAYAMA, Atsushi MIYAOKA, Takeru SUZUKI, Minoru KO
  • Publication number: 20210024887
    Abstract: Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
    Type: Application
    Filed: October 8, 2020
    Publication date: January 28, 2021
    Applicant: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Patent number: 10836997
    Abstract: Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
    Type: Grant
    Filed: March 9, 2016
    Date of Patent: November 17, 2020
    Assignee: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Publication number: 20200318074
    Abstract: A reagent for differentiating somatic cells into alveolar epithelial cells includes an NK2 homeobox family gene expression vector, and a Fox family gene expression vector.
    Type: Application
    Filed: April 19, 2018
    Publication date: October 8, 2020
    Inventors: Makoto ISHII, Tomoko BETSUYAKU, Minoru KO, Masaki IEDA, Masaya YOTSUKURA, Hisao ASAMURA, Ahmed HEGAB, Yuhki NAKATAKE, Mayumi ODA
  • Publication number: 20200048612
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being stable and highly efficient. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by attenuating differentiation resistance of a pluripotent stem cell to generate a pluripotent stem cell that actively proceeds to a differentiated cell type has been found, and thus the present invention has been completed.
    Type: Application
    Filed: October 28, 2016
    Publication date: February 13, 2020
    Applicant: KEIO UNIVERSITY
    Inventors: Minoru Ko, Tomohiko Akiyama
  • Publication number: 20190381500
    Abstract: Provided is a cell contained container including at least two concaves, wherein the concaves contain cells, wherein a number of kinds of the cells is at least two with respect to the cell contained container, and wherein a shortest distance between centers of most closely adjacent two concaves of the at least two concaves is 9.0 mm or less. In a preferable mode, the concaves contain a liquid, and a total liquid amount of the liquid with respect to the concaves is 10.0 microliters or less. In a more preferable mode, a filling accuracy in terms of a number in which the cells are contained in the concaves is 30% or lower.
    Type: Application
    Filed: June 10, 2019
    Publication date: December 19, 2019
    Inventors: Daisuke TAKAGI, Waka Lin, Takahiko Matsumoto, Manabu Seo, Tomoyuki Aratani, Takeru Suzuki, Hidekazu Yaginuma, Satoru Nagasawa, Shinnosuke Koshizuka, Tomoaki Nakayama, Minoru Ko
  • Patent number: 10392602
    Abstract: Provided is a method of producing a lacrimal gland epithelial cell from a pluripotent stem cell, a lacrimal gland epithelial cell produced by the method, and a reagent kit for inducing differentiation from a pluripotent stem cell into a lacrimal gland epithelial cell. A method of producing a lacrimal gland epithelial cell includes the following step A: Step A: a step including increasing expression of Pax6 gene in a pluripotent stem cell or transfecting PAX6 protein thereinto, and increasing expression of Foxc1 gene or Foxp1 gene in the pluripotent stem cell or transfecting FOXC1 protein or FOXP1 protein thereinto.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: August 27, 2019
    Assignee: KEIO UNIVERSITY
    Inventors: Masatoshi Hirayama, Minoru Ko
  • Publication number: 20190048320
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.
    Type: Application
    Filed: January 31, 2017
    Publication date: February 14, 2019
    Applicant: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Publication number: 20180230425
    Abstract: Provided is a method of producing a lacrimal gland epithelial cell from a pluripotent stem cell, a lacrimal gland epithelial cell produced by the method, and a reagent kit for inducing differentiation from a pluripotent stem cell into a lacrimal gland epithelial cell. A method of producing a lacrimal gland epithelial cell includes the following step A: Step A: a step including increasing expression of Pax6 gene in a pluripotent stem cell or transfecting PAX6 protein thereinto, and increasing expression of Foxc1 gene or Foxp1 gene in the pluripotent stem cell or transfecting FOXC1 protein or FOXP1 protein thereinto.
    Type: Application
    Filed: March 25, 2016
    Publication date: August 16, 2018
    Applicant: KEIO UNIVERSITY
    Inventors: Masatoshi Hirayama, Minoru Ko
  • Publication number: 20180127714
    Abstract: Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
    Type: Application
    Filed: March 9, 2016
    Publication date: May 10, 2018
    Applicant: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Publication number: 20150309026
    Abstract: The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. The methods are applicable to any type of tissue sample and can be conducted on otherwise normal tissue.
    Type: Application
    Filed: July 14, 2015
    Publication date: October 29, 2015
    Inventors: Andrew P. Feinberg, Christine A. Iacobuzio-Donahue, Dan L. Longo, Minoru Ko
  • Patent number: 9086403
    Abstract: The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. The methods are applicable to any type of tissue sample and can be conducted on otherwise normal tissue.
    Type: Grant
    Filed: June 3, 2005
    Date of Patent: July 21, 2015
    Assignees: The Johns Hopkins University, National Institutes of Health
    Inventors: Andrew P. Feinberg, Christine A. Iacobuzio-Donahue, Dan L. Longo, Minoru Ko
  • Publication number: 20060003359
    Abstract: The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. The methods are applicable to any type of tissue sample and can be conducted on otherwise normal tissue.
    Type: Application
    Filed: June 3, 2005
    Publication date: January 5, 2006
    Applicants: THE JOHNS HOPKINS UNIVERSITY, NATIONAL INSTITUTES OF HEALTH
    Inventors: Andrew Feinberg, Christine Iacobuzio-Donahue, Dan Longo, Minoru Ko