Patents by Inventor Mio Imamura

Mio Imamura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for determining mastitis using keratin and casein which are amplified by primers
    Patent number: 6287771
    Abstract: A novel primer is provided which is useful in the reverse transcription polymerase chain reaction to amplify &agr;S1-casein gene and keratin gene contained in milk. A method is also provided for using the expression ratio of their genes to examine the pathological conditions of mammalian mastitis or determine the milk protein synthesis level in mammary gland cells. The method is quick and accurate and avoids the need for excising mammary gland tissue.
    Type: Grant
    Filed: May 27, 1999
    Date of Patent: September 11, 2001
    Assignee: Snow Brand Milk Products Co., Ltd.
    Inventors: Mio Imamura, Yasuharu Itagaki, Morimasa Tanimoto
  • Method of synchronizing epithelial cells into G.sub.0 phase
    Patent number: 6143560
    Abstract: The present invention relates to a method of synchronizing epithelial cells into G.sub.0 phase, more specifically, a method of synchronizing epithelial cells into G.sub.0 phase characterized by culturing the cells in a synthetic medium for 5 days or more, wherein said synthetic medium is a medium with the lowered concentration of bovine fetal serum 5% or less and said epithelial cells is bovine mammary gland cells. Further, the present invention relates to a method of culturing cells characterized by using G.sub.0 phase synchronized cells obtained by the present invention, and a method of screening a related to differentiation and/or maturation of said cells by using G.sub.0 phase synchronized cells. The present invention is useful for easily synchronizing epithelial cells into G.sub.0 phase and for screening a factor related to differentiation and/or maturation of epithelial cells.
    Type: Grant
    Filed: October 27, 1998
    Date of Patent: November 7, 2000
    Assignee: Snow Brand Milk Products Co., Ltd.
    Inventors: Mio Imamura, Yasuharu Itagaki, Morimasa Tanimoto