Abstract: An object of the present invention is to provide an enzyme preparation that efficiently catalyzes the epimerization reaction with little byproducts. The present invention provides an enzyme preparation for catalyzing an epimerization reaction of a saccharide, which contains any of the proteins (a) to (c) mentioned below, and wherein the saccharide is any selected from the group consisting of glucose, mannose, talose and galactose: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1 or 3; (b) a protein consisting of an amino acid sequence having an amino acid sequence identity of 90% or higher to the amino acid sequence of SEQ ID NO: 1 or 3, and having an activity for catalyzing an epimerization reaction of a saccharide; and (c) a protein consisting of an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 or 3 by substitution, insertion, deletion and/or addition of one or several amino acids, and having an activity for catalyzing an epimerization reaction of a saccharide.

Abstract: An object of the present invention is to provide a protein that catalyzes an ?-1,6-glucosyl transfer reaction and can efficiently produce an ?-1,6-glucan, an enzyme preparation for producing an ?-1,6-glucan, which comprise said protein as an active ingredient, and a method for producing an ?-1,6-glucan using the enzyme preparation. The present invention provides a protein having an activity for catalyzing an ?-1,6-glucosyl transfer reaction, which is any of the proteins (a) to (c) mentioned below: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 3; (b) a protein consisting of an amino acid sequence having an amino acid sequence identity of 90% or higher to the amino acid sequence of SEQ ID NO: 3; and (c) a protein consisting of an amino acid sequence in which one or several amino acid(s) have been substituted, inserted, deleted and/or added in the amino acid sequence of SEQ ID NO: 3.