Abstract: A method for electrochemically measuring an amount of an oxidoreductase using a biosensor which includes a conductive part including two or more electrodes, and a reagent layer that is in contact with the conductive part and includes a substrate of the oxidoreductase and two or more types of mediators. A first mediator transfers, to a second mediator, electrons generated by a reaction between the substrate and the oxidoreductase, and a second mediator transfers, to the electrodes, the electrons transferred from the first mediator. The method includes bringing a sample containing an oxidoreductase into contact with a reagent layer; performing incubation for a predetermined period of time after the contact, to cause the second mediator to accumulate electrons; applying a voltage across the electrodes after the incubation; measuring an electric signal generated by the voltage application; and calculating an amount of the oxidoreductase based on the electric signal.

Abstract: A nucleic acid aptamer having binding affinity to A/H1N1pdm09 influenza virus, agents comprising the aptamer, and methods using the aptamer are provided.

Abstract: The present disclosure provides, in one aspect, a target substance detection method in which a trapping substance that indirectly binds to both an immobilization side (support side) and a detection side (reporter side), which is a method for detecting a target substance in a sample. The method includes a reaction step of reacting the sample, a support including a first binding portion, a reporter substance including a second binding portion, a first trapping substance that includes a first binding partner portion capable of binding to the first binding portion and that can bind to the target substance, and a second trapping substance that includes a second binding partner portion capable of binding to the second binding portion and that can bind to the target substance; and a detection step of detecting a signal from the reporter substance.

Abstract: A nucleic acid aptamer having binding affinity to A/H1N1pdm09 influenza virus, agents comprising the aptamer, and methods using the aptamer are provided.

Abstract: The present disclosure provides, in one aspect, a target substance detection method in which a trapping substance that indirectly binds to both an immobilization side (support side) and a detection side (reporter side), which is a method for detecting a target substance in a sample. The method includes a reaction step of reacting the sample, a support including a first binding portion, a reporter substance including a second binding portion, a first trapping substance that includes a first binding partner portion capable of binding to the first binding portion and that can bind to the target substance, and a second trapping substance that includes a second binding partner portion capable of binding to the second binding portion and that can bind to the target substance; and a detection step of detecting a signal from the reporter substance.