Patents by Inventor Misha Golynskiy
Misha Golynskiy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240141427Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: September 27, 2023Publication date: May 2, 2024Applicant: ILLUMINA, INC.Inventors: Misha Golynskiy, Rahman Rahman Pour, Jiawen Li, Ryan Craig, Hamed Tabatabaei Ghomi, Saurabh Nirantar, Hsu Myat Noe, Lin Hui Chang, Yvonne Devadas, Jing Wen Lim, Kay Klausing, Humberto Rojo, Eric Murtfeldt, Chris Garcia
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Publication number: 20240003057Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.Type: ApplicationFiled: September 14, 2023Publication date: January 4, 2024Inventors: Molly He, Michael Previte, Misha Golynskiy, Matthew William Kellinger, Sergio Peisajovich, Jonathan Mark Boutell
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Publication number: 20230383342Abstract: Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.Type: ApplicationFiled: May 30, 2023Publication date: November 30, 2023Inventors: Xiaolin Wu, Patrick McCauley, Madushani Dharmarwardana, Saurabh Nirantar, Misha Golynskiy, Xiangyuan Yang, Ramesh Neelakandan, Benedict Mackworth, Carole Anastasi, Aathavan Karunakaran, Gery M. Vessere, David Bracher
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Publication number: 20230357731Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: March 13, 2023Publication date: November 9, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 11795581Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.Type: GrantFiled: October 11, 2018Date of Patent: October 24, 2023Assignee: Illumina, Inc.Inventors: Molly He, Michael Previte, Misha Golynskiy, Matthew William Kellinger, Sergio Peisajovich, Jonathan Mark Boutell
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Publication number: 20230212534Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: December 21, 2022Publication date: July 6, 2023Applicants: ILLUMINA, INC., ILLUMINA SINGAPORE PTE LTDInventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Patent number: 11634697Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: April 9, 2021Date of Patent: April 25, 2023Assignees: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20230047225Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.Type: ApplicationFiled: June 1, 2022Publication date: February 16, 2023Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.Inventors: Misha Golynskiy, Mariko Matsuura, Saurabh Nirantar, Hamed Tabatabaei Ghomi, Seth McDonald, Ryan Craig, Sergio Peisajovich, Kyrie Johnson, Kay Klausing, Eric Murtfeldt, Alexandra Exner
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Patent number: 11560552Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: August 9, 2021Date of Patent: January 24, 2023Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20220356514Abstract: A method for detecting different analytes includes mixing different analytes with sensing probes, wherein at least some of the sensing probes are specific to respective ones of the analytes. The analytes respectively are captured by the sensing probes that are specific to those analytes. Fluorophores respectively are coupled to sensing probes that captured respective analytes. The sensing probes are mixed with beads, wherein the beads are specific to respective ones of the sensing probes, and wherein the beads include different codes identifying the analytes to which those sensing probes are specific. The sensing probes respectively are coupled to beads that are specific to those sensing probes. The beads are identified that are coupled to the sensing probes that captured analytes using at least fluorescence from the fluorophores coupled to those sensing probes. The analytes that are captured are identified.Type: ApplicationFiled: October 12, 2020Publication date: November 10, 2022Applicants: ILLUMINA, INC., ILLUMINA SINGAPORE PTE. LTD., ILLUMINA CAMBRIDGE LIMITEDInventors: Sarah SHULTZABERGER, Jeffrey BRODIN, Yin Nah TEO, Suzanne ROHRBACK, Rebecca MACLEOD, Rigo PANTOJA, Allen ECKHARDT, Jeffrey FISHER, Xiangyuan YANG, Kaitlin PUGLIESE, Misha GOLYNSKIY, Xiaolin WU, Seth MCDONALD
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Publication number: 20220056424Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: August 9, 2021Publication date: February 24, 2022Applicants: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20210371833Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.Type: ApplicationFiled: April 2, 2021Publication date: December 2, 2021Applicant: ILLUMINA, INC.Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
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Publication number: 20210348141Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: April 9, 2021Publication date: November 11, 2021Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 11104888Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: October 31, 2019Date of Patent: August 31, 2021Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Patent number: 11001816Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: GrantFiled: December 4, 2019Date of Patent: May 11, 2021Assignees: Illumina, Inc., Illumina Singapore PTE. LTD., Illumina Cambridge LimitedInventors: Kay Klausing, Hamed Tabatabaei Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Patent number: 10995324Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.Type: GrantFiled: May 6, 2019Date of Patent: May 4, 2021Assignee: Illumina, Inc.Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
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Publication number: 20200181587Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: December 4, 2019Publication date: June 11, 2020Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Kay Klausing, Hamed Tabatabael Ghomi, Misha Golynskiy, Saurabh Nirantar, Seth McDonald, Sergio Peisajovich
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Publication number: 20200131484Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.Type: ApplicationFiled: October 31, 2019Publication date: April 30, 2020Applicants: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
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Publication number: 20190256828Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.Type: ApplicationFiled: May 6, 2019Publication date: August 22, 2019Applicant: Illumina, Inc.Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
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Patent number: 10329545Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.Type: GrantFiled: November 13, 2015Date of Patent: June 25, 2019Assignee: ILLUMINA, INC.Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich