Abstract: The present invention relates to a method for engineering a protein in a host cell, comprising the following steps: identifying a protein of interest and introducing the coding sequence of the protein of interest into the genome of the host cell; screening for hotspots for amino acid mutations in the protein of interest; generating a set of specific guide RNAs and libraries of homologous recombination template that generate mutations at the desired sites within the protein coding region; introducing the guide RNA and the library of homologous recombination template into the host cell, thereby producing mutated protein coding regions; screening to select for cells that express the protein of interest with desired activity and/or property from the mutated protein coding regions, thereby providing an engineered protein.

Abstract: The invention relates to an enzyme product comprising a beta-galactosidase product. The invention provides that the beta-galactosidase product has an inactive arylsulphatase.

Abstract: Suggested is a method for engineering and/or editing the genome of prokaryotes encompassing the following steps: (i) providing a culture of prokaryotic cells, (ii) preparing a vector comprising an expression system encompassing at least one programmable DNA-binding and -cleaving protein, (iii) introducing said vector into said prokaryotic cells to target a specific DNA sequence in the genome of said prokaryotic cells.

Abstract: The present invention relates to a method for enriching cells within a cell population, wherein a gene of interest is genome edited, said method comprising (a) introducing into the cells within the cell population one or more nucleic acid molecules, said one or more nucleic acid molecules encoding in expressible form (i) a CRISPR nuclease, (ii) a guide RNA for knocking-out the leucine-rich repeat-containing protein 8 A (LRRC8A), LRRC8B, LRRC8C, LRRC8D or LRRC8E gene or a guide RNA for knocking-out an innexin gene, and (iii) a guide RNA for editing the gene of interest; or (a?) introducing into the cells within the cell population (i) a CRISPR nuclease, and one or more nucleic acid molecules, said one or more nucleic acid molecules encoding in expressible form (ii) a guide RNA for knocking-out the leucine-rich repeat-containing protein 8 A (LRRC8A), LRRC8B, LRRC8C, LRRC8D or LRRC8E gene or a guide RNA for knocking-out an innexin gene, and (iii) a guide RNA for editing the gene of interest; or (a?) introducting