Abstract: The present disclosure provides devices, systems, and methods for capturing and analyzing behavioral and physiological data of subjects for treatment, modification, and manipulation discovery. In some embodiments, a method for classifying a drug is provided. The method includes obtaining observational data concerning an animal subject to which the drug is administered, the observational data acquired using an enclosure for the animal subject, the enclosure instrumented with at least one sensing device. The method also includes extracting features by applying the observational data to a machine-learning feature-extraction component. The method further includes predicting a class label of the drug by applying the features to a machine-learning classifier component, the machine-learning classifier component trained to predict the class label of the drug from, at least in part, the features. The method further includes providing an indication of the class label.

Abstract: The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Abstract: Various blanks and constructs formed therefrom are provided. The various constructs include features for supporting a food item at an elevated position to enhance the heating, browning, and/or crisping of the food item in a microwave oven.