Abstract: Provided are a method of detecting a target substance, by which the detection sensitivity in the PALSAR method can be improved and multiple genes can be simultaneously detected, and a kit for detection.

Abstract: Provided is a method of detecting a target substance by which the target substance can be detected with efficiency and a high sensitivity, and in a simple manner, a target substance detection polymer used in the method, and a method of forming the polymer. The method of detecting a target substance includes the steps of: (A) forming a target substance detection polymer by causing multiple kinds of nucleic acid probes for forming a polymer to react with a binding probe having a region capable of binding to the target substance and a region capable of binding to at least one of the nucleic acid probes in a solution; (B) binding the target substance detection polymer and the target substance; and (C) detecting the target substance detection polymer to which the target substance is bound.

Abstract: Provided are a method of forming a signal probe-polymer which makes it possible to form a polymer efficiently and quantitatively, a signal probe-polymer formed by the method, oligonucleotide probes for use in the method, and a method of detecting target analyte having high sensitivity and excellent quantitative capability. The method of forming a signal probe-polymer comprises reacting a plurality of pairs of oligonucleotide probes with each other to form a polymer, a first probe of the pair of oligonucleotide probes comprising three nucleic acid regions of X, Y, and Z regions, located in the stated order from the 5?-terminal and a second probe comprising three nucleic acid regions of X?, Y?, and Z? regions, located in the stated order from the 5?-terminal, wherein each region of the oligonucleotide probes has a length of from 13 to 15 bases.

Abstract: To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on).

Abstract: Provided are a method of detecting target genes capable of increasing sensitivity in a Palsar method and of simultaneously detecting multiple genes, an assist probe to be used in the above method, and a method of forming a signal probe polymer by using the assist probe. The method of detecting target genes includes: forming a signal probe polymer by using a first probe having a nucleic acid region X, a nucleic acid region Y and a nucleic acid region Z in the stated order from the 5? end, a second probe having a nucleic acid region X?, a nucleic acid region Y? and a nucleic acid region Z? in the stated order from the 5? end, and an assist probe having a plurality of the same nucleic acid regions as in the first probe and a target region capable of hybridizing with a target gene.

Abstract: Provided are a method of forming a probe-polymer capable of readily and efficiently forming a polymer of a nucleic acid probe, a probe-polymer formed by the method, and a novel nucleic acid probe used in the method, and a detection method for a target analyte capable of sensitively and readily detecting the target analyte. The nucleic acid probe is formed by including three or more nucleic acid regions, in which each of the nucleic acid regions includes a first region and a second region complementary to the first region, the both regions being adjacent to each other. The polymer of the nucleic acid probe is formed by reacting the nucleic acid probe.

Abstract: Provided are a method of forming a signal probe-polymer which makes it possible to form a polymer efficiently and quantitatively, a signal probe-polymer formed by the method, oligonucleotide probes for use in the method, and a method of detecting target analyte having high sensitivity and excellent quantitative capability. The method of forming a signal probe-polymer comprises reacting a plurality of pairs of oligonucleotide probes with each other to form a polymer, a first probe of the pair of oligonucleotide probes comprising three nucleic acid regions of X, Y, and Z regions, located in the stated order from the 5?-terminal and a second probe comprising three nucleic acid regions of X?, Y?, and Z? regions, located in the stated order from the 5?-terminal, wherein each region of the oligonucleotide probes has a length of from 13 to 15 bases.

Abstract: A hybridization method is provided in which an efficient hybridization reaction can be carried out. Further, there are provided, using this hybridization method, a method for detecting a target gene with high sensitivity and a signal amplifying method for markedly improving the detection sensitivity of the target gene. There is provided a hybridization method comprising the use of oligonucleotides in a reaction solution, the method comprising forming partially a reaction temperature region in the reaction solution and performing a hybridization reaction in the reaction temperature region.

Abstract: Provided are a method of detecting a target substance whereby the detection sensitivity in the PALSAR method can be improved and multiple genes can be simultaneously detected, and a kit for detection. The method of detecting a target substance is a method of detecting a target substance by forming a signal probe polymer using one or more sets of paired dimer-forming probes for forming dimer probes or dimer probes, one or more kinds of crosslinking probes, and one or more kinds of assist probes, in which a dimer-forming probe includes a 5?-side region, a central region, and a 3?-side region, a crosslinking probe includes two regions, i.e.

Abstract: Provided are a method of detecting a target substance, by which the detection sensitivity in the PALSAR method can be improved and multiple genes can be simultaneously detected, and a kit for detection.

Abstract: There is provided a signal amplification method for detecting a mutated gene, which can increase the detection sensitivity of mutated genes on a DNA chip according to the PALSAR method, can establish efficient signal amplification and can establish simple detection by contriving design of oligonucleotide probes for use in the PALSAR method. The signal amplification method comprises a ligation reaction with a DNA ligase and a self-assembly reaction which forms a double-stranded self-assembly substance having a regular higher-order structure of oligonucleotides, wherein the detection sensitivity of the mutated gene on a DNA chip is improved.

Abstract: It is intended to provide a method, in which the sensitivity in detecting a target analyte on a microsphere can be elevated and plural items can be detected at the same time. The presence of at least one target analyte in a sample is detected by bonding a microsphere having a fluorescent substance on the surface and the target analyte to a self assembly substance formed by using two oligonucleotide probes having complementary base sequence regions which are hybridizable with each other via the target analyte, thus forming a self assembly substance-bonded particle, and then analyzing the self assembly substance-bonded particle.

Abstract: It is intended to provide: a sampling device for quantitatively sampling a liquid sample having a high viscosity such as sputum; an auxiliary jig therefor; an agent for homogenizing sputum by which sputum can be quickly treated under mild conditions and a microbe contained in the sputum can be detected at an improved stability; a homogenization device; a homogenization method; a method of detecting a microbe by using the above method; a separation device for conveniently, quickly and efficiently collecting a microbe contained in sputum; a method of collecting a microbe; and a method of detecting a microbe by using the above method.

Abstract: Provided are a signal amplification method of improving signal sensitivity, qualifying properties and handling property in detection of a target gene by using a PALSAR method, a method of detecting a target gene by using the method, and an oligonucleotide probe to be used in the method. A signal amplification method in detection of a target gene using a polymer formed by the use of a plurality of kinds of oligonucleotide probes having complementary base sequence regions capable of hybridizing with each other, including labeling at least one of the plurality of kinds of oligonucleotide probes with acridinium ester for detection.

Abstract: A hybridization method is provided in which an efficient hybridization reaction can be carried out. Further, there are provided, using this hybridization method, a method for detecting a target gene with high sensitivity and a signal amplifying method for markedly improving the detection sensitivity of the target gene. There is provided a hybridization method comprising the use of oligonucleotides in a reaction solution, the method comprising forming partially a reaction temperature region in the reaction solution and performing a hybridization reaction in the reaction temperature region.

Abstract: To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on).

Abstract: Provided are a method of detecting target genes capable of increasing sensitivity in a Palsar method and of simultaneously detecting multiple genes, an assist probe to be used in the above method, and a method of forming a signal probe polymer by using the assist probe. The method of detecting target genes includes: forming a signal probe polymer by using a first probe having a nucleic acid region X, a nucleic acid region Y and a nucleic acid region Z in the stated order from the 5? end, a second probe having a nucleic acid region X?, a nucleic acid region Y? and a nucleic acid region Z? in the stated order from the 5? end, and an assist probe having a plurality of the same nucleic acid regions as in the first probe and a target region capable of hybridizing with a target gene.

Abstract: There is provided a method for forming a self-assembly substance using oligonucleotides without using special instruments or complicated procedures, an self-assembly substance formed by the method for forming the self-assembly substance, and a method for detecting an amplified target gene at a low cost and in a simple way by making use of the method for forming the self-assembly substance. In the method for forming the self-assembly substance using a self-assembly reaction of oligonucleotides, the oligonucleotides comprise oligonucleotides synthesized by a gene amplification reaction. The oligonucleotides synthesized by the gene amplification reaction are detected by forming a self-assembly substance by the use of the method for forming the self-assembly substance of oligonucleotides, and by detecting the formed self-assembly substance.

Abstract: There is provided a method for forming a self-assembly substance using oligonucleotides without using special instruments or complicated procedures, an self-assembly substance formed by the method for forming the self-assembly substance, and a method for detecting an amplified target gene at a low cost and in a simple way by making use of the method for forming the self-assembly substance. In the method for forming the self-assembly substance using a self-assembly reaction of oligonucleotides, the oligonucleotides comprise oligonucleotides synthesized by a gene amplification reaction. The oligonucleotides synthesized by the gene amplification reaction are detected by forming a self-assembly substance by the use of the method for forming the self-assembly substance of oligonucleotides, and by detecting the formed self-assembly substance.

Abstract: The present invention provides a novel method for forming a self-assembly substance of probes making it possible to shorten a reaction time required for formation of a self-assembly substance and also to increase regions of probes available for detection of a target gene by previously preparing a plurality of dimer-probes and increasing the types. This method comprises the steps of: providing plural groups, wherein each group includes a pair of dimer forming probes containing a pair of oligonucleotides, each oligonucleotide having three regions of a 3? side region, a mid-region and a 5? side region, in which the mid-regions of the oligonucleotides have base sequences complementary to each other, and the 3? side regions and the 5? side regions of the oligonucleotides have base sequences not complementary to each other; hybridizing a plurality of pairs of the dimer forming probes of the plural groups; and forming a double-stranded self-assembly substance by self-assembly of the oligonucleotides.