Patents by Inventor Mitsuhisa Manabe

Mitsuhisa Manabe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20120149047
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: July 11, 2011
    Publication date: June 14, 2012
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Publication number: 20100227309
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: May 18, 2010
    Publication date: September 9, 2010
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Publication number: 20090226945
    Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.
    Type: Application
    Filed: May 18, 2009
    Publication date: September 10, 2009
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro NAKAMURA, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
  • Publication number: 20080131911
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholesterols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: February 4, 2008
    Publication date: June 5, 2008
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro NAKAMURA, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Patent number: 7335483
    Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.
    Type: Grant
    Filed: July 26, 2004
    Date of Patent: February 26, 2008
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
  • Publication number: 20060183179
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: April 7, 2006
    Publication date: August 17, 2006
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Publication number: 20050009125
    Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.
    Type: Application
    Filed: July 26, 2004
    Publication date: January 13, 2005
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
  • Publication number: 20040265939
    Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.
    Type: Application
    Filed: July 26, 2004
    Publication date: December 30, 2004
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
  • Patent number: 6835543
    Abstract: An immunoassay for detecting an antigen in a sample, by: (a) sequentially contacting the sample with (i) a first antibody which is capable of specifically binding to a first binding site on the antigen, and then (ii) a second antibody which is capable of specifically binding to a second binding site on the antigen, thereby forming, when the antigen is present in the sample, an agglutinate comprising the first antibody, the antigen, and the second antibody; followed by (b) optically measuring the amount of the agglutinate.
    Type: Grant
    Filed: July 11, 1997
    Date of Patent: December 28, 2004
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Kazunori Saitoh, Mitsuhisa Manabe
  • Patent number: 6818414
    Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: November 16, 2004
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
  • Publication number: 20040219623
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: June 4, 2004
    Publication date: November 4, 2004
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Patent number: 6764828
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Grant
    Filed: October 9, 2001
    Date of Patent: July 20, 2004
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Publication number: 20020015975
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density—and very low density—cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Application
    Filed: October 9, 2001
    Publication date: February 7, 2002
    Applicant: DAIICHI PURE CHEMICALS CO., LTD.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Patent number: 6333166
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Grant
    Filed: February 22, 2000
    Date of Patent: December 25, 2001
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Patent number: 6248545
    Abstract: Disclosed herein is an assay of a denatured lipoprotein, in which the denatured site of the denatured lipoprotein contained in a vital sample is exposed to the surface of its lipoprotein particle upon the reaction of an antibody, which recognizes the denatured lipoprotein, with the vital sample containing the denatured lipoprotein.
    Type: Grant
    Filed: October 14, 1997
    Date of Patent: June 19, 2001
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Akira Kondo, Naoko Toda, Noriko Kobayashi, Masayuki Nozawa, Mitsuhisa Manabe
  • Patent number: 6057118
    Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
    Type: Grant
    Filed: November 23, 1998
    Date of Patent: May 2, 2000
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
  • Patent number: 5773304
    Abstract: A method for quantitatively determining cholesterol in high density lipoproteins, in which, prior to the determination of cholesterol by an enzymatic method, a surfactant and a substance which forms a complex with lipoproteins other than high density lipoproteins are added to a sample containing lipoproteins.The method does not require any pretreatments such as centrifugal separation. With a simple operation, cholesterol in HDLs can be measured effectively. Also, this method can be adopted in a variety of automated analyzers, and thus is very useful in the field of clinical assays.
    Type: Grant
    Filed: September 19, 1996
    Date of Patent: June 30, 1998
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Koichi Hino, Mitsuhiro Nakamura, Mitsuhisa Manabe
  • Patent number: 4933277
    Abstract: A method for quantitative analysis of hydrogen peroxide at a high precision is disclosed. The method comprises reacting a divalent cobalt compound with hydrogen peroxide in the presence of peroxidase or a substance similar to peroxidase to produce a trivalent cobalt compound, reacting the trivalent cobalt compound thus produced with a trivalent cobalt indicator to produced a colored complex, and subjecting the colored complex to colorimetric quantitative analysis. The method enables various enzymatic activities producing hydrogen peroxide by the reaction with substrates as well as the amount or activity of substances involving the known enzymatic reactions linked with such enzymatic reactions, of enzymes and coenzymes, to be quantitatively determined very easily at a high sensitivity at a wavelength of above 600 nm without the interference of hemoglobin, bilirubin, or turbidity.
    Type: Grant
    Filed: October 26, 1988
    Date of Patent: June 12, 1990
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Toshikatsu Abe, Mitsuhisa Manabe, Masayuki Nozawa, Atsushi Izumi, Fumio Masumi, Akemichi Maki