Patents by Inventor Mitsuhisa Manabe
Mitsuhisa Manabe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120149047Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: July 11, 2011Publication date: June 14, 2012Applicant: SEKISUI MEDICAL CO., LTD.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Publication number: 20100227309Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: May 18, 2010Publication date: September 9, 2010Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Publication number: 20090226945Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: ApplicationFiled: May 18, 2009Publication date: September 10, 2009Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro NAKAMURA, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Publication number: 20080131911Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholesterols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: February 4, 2008Publication date: June 5, 2008Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro NAKAMURA, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Patent number: 7335483Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: GrantFiled: July 26, 2004Date of Patent: February 26, 2008Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Publication number: 20060183179Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: April 7, 2006Publication date: August 17, 2006Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Publication number: 20050009125Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: ApplicationFiled: July 26, 2004Publication date: January 13, 2005Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Publication number: 20040265939Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: ApplicationFiled: July 26, 2004Publication date: December 30, 2004Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Patent number: 6835543Abstract: An immunoassay for detecting an antigen in a sample, by: (a) sequentially contacting the sample with (i) a first antibody which is capable of specifically binding to a first binding site on the antigen, and then (ii) a second antibody which is capable of specifically binding to a second binding site on the antigen, thereby forming, when the antigen is present in the sample, an agglutinate comprising the first antibody, the antigen, and the second antibody; followed by (b) optically measuring the amount of the agglutinate.Type: GrantFiled: July 11, 1997Date of Patent: December 28, 2004Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Kazunori Saitoh, Mitsuhisa Manabe
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Patent number: 6818414Abstract: A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.Type: GrantFiled: December 21, 2001Date of Patent: November 16, 2004Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Yuriko Taniguchi, Mitsuhisa Manabe, Mitsuaki Yamamoto
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Publication number: 20040219623Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: June 4, 2004Publication date: November 4, 2004Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Patent number: 6764828Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: GrantFiled: October 9, 2001Date of Patent: July 20, 2004Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Publication number: 20020015975Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density—and very low density—cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: ApplicationFiled: October 9, 2001Publication date: February 7, 2002Applicant: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Patent number: 6333166Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: GrantFiled: February 22, 2000Date of Patent: December 25, 2001Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Patent number: 6248545Abstract: Disclosed herein is an assay of a denatured lipoprotein, in which the denatured site of the denatured lipoprotein contained in a vital sample is exposed to the surface of its lipoprotein particle upon the reaction of an antibody, which recognizes the denatured lipoprotein, with the vital sample containing the denatured lipoprotein.Type: GrantFiled: October 14, 1997Date of Patent: June 19, 2001Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Akira Kondo, Naoko Toda, Noriko Kobayashi, Masayuki Nozawa, Mitsuhisa Manabe
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Patent number: 6057118Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.Type: GrantFiled: November 23, 1998Date of Patent: May 2, 2000Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Mitsuhiro Nakamura, Kazuo Nakanishi, Koichi Hino, Mitsuhisa Manabe
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Patent number: 5773304Abstract: A method for quantitatively determining cholesterol in high density lipoproteins, in which, prior to the determination of cholesterol by an enzymatic method, a surfactant and a substance which forms a complex with lipoproteins other than high density lipoproteins are added to a sample containing lipoproteins.The method does not require any pretreatments such as centrifugal separation. With a simple operation, cholesterol in HDLs can be measured effectively. Also, this method can be adopted in a variety of automated analyzers, and thus is very useful in the field of clinical assays.Type: GrantFiled: September 19, 1996Date of Patent: June 30, 1998Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Koichi Hino, Mitsuhiro Nakamura, Mitsuhisa Manabe
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Patent number: 4933277Abstract: A method for quantitative analysis of hydrogen peroxide at a high precision is disclosed. The method comprises reacting a divalent cobalt compound with hydrogen peroxide in the presence of peroxidase or a substance similar to peroxidase to produce a trivalent cobalt compound, reacting the trivalent cobalt compound thus produced with a trivalent cobalt indicator to produced a colored complex, and subjecting the colored complex to colorimetric quantitative analysis. The method enables various enzymatic activities producing hydrogen peroxide by the reaction with substrates as well as the amount or activity of substances involving the known enzymatic reactions linked with such enzymatic reactions, of enzymes and coenzymes, to be quantitatively determined very easily at a high sensitivity at a wavelength of above 600 nm without the interference of hemoglobin, bilirubin, or turbidity.Type: GrantFiled: October 26, 1988Date of Patent: June 12, 1990Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Toshikatsu Abe, Mitsuhisa Manabe, Masayuki Nozawa, Atsushi Izumi, Fumio Masumi, Akemichi Maki