Patents by Inventor Mitsuo Kawase
Mitsuo Kawase has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180119211Abstract: The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a DNA polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected.Type: ApplicationFiled: November 22, 2017Publication date: May 3, 2018Applicant: NGK INSULATORS, LTD.Inventors: Kousuke Niwa, Toshikazu Hirota, Mitsuo Kawase
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Publication number: 20160032367Abstract: An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.Type: ApplicationFiled: October 14, 2015Publication date: February 4, 2016Applicant: NGK INSULATORS, LTD.Inventors: Mitsuo Kawase, Toshikazu Hirota, Kousuke Niwa
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Patent number: 9175339Abstract: An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.Type: GrantFiled: October 26, 2010Date of Patent: November 3, 2015Assignee: NGK Insulators, Ltd.Inventors: Mitsuo Kawase, Toshikazu Hirota, Kousuke Niwa
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Publication number: 20140206567Abstract: The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a DNA polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected.Type: ApplicationFiled: March 13, 2014Publication date: July 24, 2014Applicant: NGK INSULATORS, LTD.Inventors: Kousuke Niwa, Toshikazu Hirota, Mitsuo Kawase
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Publication number: 20120196765Abstract: A method of detecting or analyzing a target sequence in a genomic DNA by using a capture probe immobilized on a solid carrier includes: bringing the target nucleic acid into contact with a first query probe that has a sequence complementary to a portion of the target sequence or to a sequence adjacent to the portion and a second query probe that has a sequence complementary to another portion of the target sequence or to a sequence adjacent to the another portion and that has a recognition sequence complementary to a portion of the capture probe; acquiring a ligated molecule by ligating the first query probe and the second query probe that are hybridized to the target nucleic acid; bringing the ligated molecule into contact with the capture probe on the solid carrier and then capturing the ligated molecule on the solid carrier by hybridizing the capture probe with the recognition sequence in the ligated molecule; and detecting the captured ligated molecule.Type: ApplicationFiled: July 9, 2010Publication date: August 2, 2012Inventors: Mitsuo Kawase, Toshikazu Hirota, Kousuke Niwa
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Publication number: 20120178648Abstract: An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.Type: ApplicationFiled: October 26, 2010Publication date: July 12, 2012Inventors: Mitsuo Kawase, Toshikazu Hirota, Kousuke Niwa
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Publication number: 20110046015Abstract: Disclosed is a peptide immobilization technique which can achieve the immobilization of a satisfactory quantity of a peptide on a solid support. In the immobilization of a peptide on a solid support, a surfactant is allowed to coexist on the solid support together with the peptide. In this manner, the quantity of the peptide immobilized on the solid support can be increased.Type: ApplicationFiled: January 29, 2009Publication date: February 24, 2011Inventors: Hiroyuki Honda, Tsutomu Kawabe, Mina Okochi, Miyoko Matsushima, Naoki Matsumoto, Mitsuo Kawase, Yasuko Yoshida, Tomokazu Takase
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Publication number: 20070292853Abstract: A DNA array for detecting a single nucleotide polymorphism of a gene, which comprises, on a solid support, a first probe spots group consisting of one or more probe spots each containing one or more probes hybridizable with a polynucleotide of the gene, in which the probes are exactly complementary to the first polymorphism pattern of the gene, and a second probe spots group consisting of one or more probe spots each containing one or more probes hybridizable with the polynucleotide of the gene, in which the probes are exactly complementary to the second polymorphism pattern of the gene, wherein probe lengths in the probe spots is different from each other. This DNA array enables more exact SNP detection.Type: ApplicationFiled: March 18, 2005Publication date: December 20, 2007Applicants: TOYOBO CO., LTD., NGK INSULATORS, LTD.Inventors: Mitsuo Kawase, Yasuko Yoshida, Kazunari Yamada, Yutaka Takarada, Kouzo Hashimoto
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Publication number: 20070238870Abstract: The hybridization device of the invention aims to attain a hybridization reaction of high reproducibility. A hybridization device 2 for a hybridization reaction of nucleic acid has a cover member 10 that defines a cavity 12, which includes a nucleic acid fixation area 6 of a substrate 4 for fixation of a nucleic acid probe and has capacity for storage of a liquid for the hybridization reaction therein. At least part of an area exposed to inside of the cavity 12 forms a hydrophobic region 18. Adequate control of the surface characteristic of the area exposed to the cavity for implementing the hybridization reaction desirably enhances the signal intensity and reduces a variation in signal intensity, thus attaining the hybridization reaction of high reproducibility.Type: ApplicationFiled: July 29, 2005Publication date: October 11, 2007Applicant: NGK Insulators, Ltd.Inventors: Mitsuo Kawase, Yasuko Yoshida, Kazunari Yamada, Tomokazu Takase
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Patent number: 7052842Abstract: A method for hybridizing nucleic acids, which includes an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with fluorescence or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step. In the annealing step, the complementary double strand is formed by performing a temperature gradient processing performed from a high temperature area to a low temperature area, and in the enzyme treatment step, a noncomplementary nucleic acid portion contained in the complementary double stand is recognized and cleaved by adding endonuclease. This method has high measurement sensitivity and the operation is simple.Type: GrantFiled: March 1, 2002Date of Patent: May 30, 2006Assignee: NGK Insulators, Ltd.Inventors: Mitsuo Kawase, Kazunari Yamada, Isao Miyazaki, Chiho Matsuda
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Patent number: 6395717Abstract: Therapeutic drugs for endotoxin blood symptom and multi-organ failure induced by it is provided, which therapeutic during are composed of sialic acid its salt, polymers of sialic acid or a salt of the polymer as effective components and have high therapeutic effects for shock death and organ failure induced by endotoxin as well as high safety, and are effective for treatment of endotoxin-shock, and organ failure.Type: GrantFiled: January 23, 1997Date of Patent: May 28, 2002Assignees: NGK Insulatirs, Ltd., Biseiken Co., Ltd.Inventors: Michio Ohta, Takaaki Hasegawa, Masayuki Nadai, Yasuko Yoshida, Mitsuo Kawase, Tadahiko Inukai
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Patent number: 6182659Abstract: A virus-removing filter using, as a virus-capturing body, at least one kind of Streptococcus agalactiae type II culture and Type II polysaccharide.Type: GrantFiled: June 9, 1998Date of Patent: February 6, 2001Assignee: NGK Insulators, Ltd.Inventors: Mitsuo Kawase, Yuji Kawase, Kazunari Yamada, Yasuo Suzuki
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Patent number: 6083931Abstract: The present invention is aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells when used alone. In addition, the present invention is also aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells while causing a small side effect and very small toxicity even for an extended time of administration. The cancer metastasis-inhibiting anticancer agent according to the present invention is characterized by containing sialic acid, its salt, a polymer of sialic acid or a salt of the polymer as an effective ingredient.Type: GrantFiled: December 9, 1997Date of Patent: July 4, 2000Assignees: NGK Insulators, Ltd., Biseiken Co., Ltd.Inventors: Takaaki Hasegawa, Kenichi Miyamoto, Mitsuo Kawase, Yasuko Yoshida, Tadahiko Inukai
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Patent number: 5939404Abstract: The present invention relates to the technology for inhibiting the cancer metastasis, and is aimed at the provision of a pharmaceutical composition for effectively inhibiting the metastasis of the cancer. A cancer metastasis inhibitor according to the present invention is characterized by containing a sugar chain separated from a surface of a Streptococcus agalactiae Ia type or Ib type as a main ingredient. Since the surface sugar chains of the Streptococcus agalactiae Ia type or Ib type have structures similar to surface sugar chains of the cancer cells, the former surface sugar chains adhere to E-selectin appearing in an intravascular endothelial cell, competitively inhibit the adhesion between intravascular endothelial cells and the cancer cells, and effectively prevent of the metastasis of the cancer, when the sugar chains exist in blood of a patient.Type: GrantFiled: August 19, 1997Date of Patent: August 17, 1999Assignee: NGK Insulators, Ltd.Inventors: Shinji Iijima, Katsuhide Miyake, Shin Yamamoto, Yasuko Yoshida, Mitsuo Kawase
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Patent number: 5851395Abstract: A virus-removing filter using, as a virus-capturing body, at least one kind of sialic acid, a sialic acid derivative, and sugars, glycoproteins and glycolipids containing sialic acid and/or the sialic acid derivative.Type: GrantFiled: December 20, 1996Date of Patent: December 22, 1998Inventors: Mitsuo Kawase, Yuji Kawase, Kazunari Yamada, Kazukiyo Kobayashi, Yasuo Suzuki
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Patent number: 5143847Abstract: An enzyme-fixed bioreactor, including a reaction column, enzyme-fixed catalyst particles uniformly and densely filled in the reaction column, the catalyst particles being composed of carrier sepiolite particles consisting essentially of sepiolite and an enzyme carried on the surface of the carrier sepiolite particles. The bioreactor has stable heat resistant and chemical properties, high productivity, and is economically superior to prior art, without fear of destruction of the catalyst, short path, and clogging in the reaction column.Type: GrantFiled: November 27, 1989Date of Patent: September 1, 1992Assignee: NGK Insulators, Ltd.Inventors: Mitsuo Kawase, Yasuko Yoshida, Hitoshi Yonekawa
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Patent number: 5100553Abstract: Proliferation activity of methane bacteria in organic waste water can be improved by maintaining the ratio of the amounts of nickel, iron and cobalt contained in the waste water to the BOD thereof to not lower than certain values, and various organic waste waters, such as waste liquor formed during the production of beer and the like, can be treated in a high BOD removal ratio of not lower than 90% while maintaining a high BOD load of not lower than 10 kg/m.sup.3 .multidot.day.Type: GrantFiled: August 20, 1990Date of Patent: March 31, 1992Assignee: NGK Insulators, Ltd.Inventors: Tadashi Nomura, Mitsuo Kawase, Naoki Murata
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Patent number: 4872439Abstract: A device for preventing an outflow of a fuel vapor from a fuel tank comprising a breather pipe which connects the upper interior of the fuel tank to a charcoal canister. A float-valve assembly is mounted on the upper wall of the fuel tank. This assembly comprises a breather passage connected to the charcoal canister via the breather pipe, a normally closed solenoid valve arranged in the breather passage, and a float normally allowing the breather passage to be open to the interior of the fuel tank. When the replenishment of the fuel tank is carried out, the solenoid valve is opened to feed fuel vapor in the fuel tank into the charcoal canister.Type: GrantFiled: February 1, 1988Date of Patent: October 10, 1989Assignees: Toyota Jidosha Kabushiki Kaisha, Aisan Industry Co., Ltd., Jeco Co.,Ltd.Inventors: Hidefumi Sonoda, Eiji Mori, Mitsuo Kawase, Kazuhiro Yoneshige, Akira Takahashi, Hirokazu Komiya, Akira Tanaka
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Patent number: 4723791Abstract: A suspension of a vehicle includes a set of suspension arms each supporting a wheel, a subframe pivotably connected on its left and right sides with the respective suspension arms, the subframe having on its each side portions forward and rearward of a rotary axis of the wheel and six elastic mounts. The subframe is coupled on each side thereof with a car body through two of the elastic mounts disposed at lateral interval in one of the portion and one elastic mount disposed in the other of the portions.Type: GrantFiled: October 21, 1986Date of Patent: February 9, 1988Assignee: Toyota Jidosha Kabushiki KaishaInventors: Kiyokatsu Miura, Nobuo Hiraiwa, Hajime Naruse, Shogo Tanaka, Hisaomi Kumai, Mitsuo Kawase, Naoto Kitagawa
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Patent number: 4257623Abstract: A supporting structure for a suspension member on a vehicle includes a front cushion and a rear cushion provided between the suspension member and the body of the vehicle. The front cushion is designed with a small spring rate against a load acting longitudinally on the vehicle and a vertical load on the vehicle. The rear cushion provides a large spring rate against a vertical load on the vehicle.Type: GrantFiled: May 23, 1979Date of Patent: March 24, 1981Assignee: Toyoda Jidosha Kogyo Kabushiki KaishaInventors: Mitsuo Kawase, Hiroyuki Watanabe