Patents by Inventor Mitsuo Oshimura
Mitsuo Oshimura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230407268Abstract: An object of the present invention is to provide a method for producing a reversibly immortalized cell which can allow a cell into which an immortalizing gene is introduced to proliferate over a long period without damaging the chromosome of the cell, and is capable of removing the immortalizing gene, and to provide a method for obtaining a large amount of a reversibly immortalized cell that can be cloned and has stable quality. The present invention provides a method for producing a reversibly immortalized cell, comprising the steps of: introducing a chromosomally non-integrated RNA virus vector loaded with one or two or more immortalizing gene(s), selected from the group consisting of Bmi-1 gene, TERT gene, and SV40T gene, into a mammalian cell so that the immortalizing gene is expressed in the cell; and culturing the obtained cell for proliferation.Type: ApplicationFiled: November 5, 2021Publication date: December 21, 2023Applicants: Trans Chromosomics, Inc., NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITYInventors: Mitsuo OSHIMURA, Toshiaki TABATA, Yasuhiro KAZUKI, Narumi UNO
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Patent number: 11825817Abstract: In this application, the provided are: a Down syndrome rat model characterized in that a rat gene homologous to at least one gene present on a human chromosome 21 or fragment thereof is a trisomy and is transmittable to progeny; or a Down syndrome rat model characterized in that it comprises a human chromosome 21 or fragment thereof, or an exogenous rat chromosome or fragment thereof on which a rat gene homologous to the human chromosome 21 or fragment thereof is present, wherein at least one gene on the human chromosome 21 or fragment thereof or on the exogenous rat chromosome or fragment thereof is added to endogenous rat genes homologous to the at least gene so as to become a trisomy and to be transmittable to progeny: and a method for producing the Down syndrome rat model.Type: GrantFiled: September 28, 2017Date of Patent: November 28, 2023Assignees: National University Corporation Tottori University, Trans Chromosomics, Inc.Inventors: Yasuhiro Kazuki, Mitsuo Oshimura
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Publication number: 20210163896Abstract: This application provides a method for preparing human cell-derived microcells from human cells comprising DNA of interest, comprising: a step of culturing human cells in a medium containing at least one micronucleus inducer selected from the group consisting of microtubule polymerization inhibitors (excepting colcemid), microtubule depolymerization inhibitors, and spindle checkpoint inhibitors, thereby to produce human cell-derived microcells; and a step of collecting human cell-derived microcells comprising the DNA of interest, and a method for preparing human cells comprising DNA of interest by use of the method, which can be also applied to non-human animal cells.Type: ApplicationFiled: October 10, 2019Publication date: June 3, 2021Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, Trans Chromosomics, Inc.Inventors: Yasuhiro KAZUKI, Narumi UNO, Mitsuo OSHIMURA
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Publication number: 20210123027Abstract: The present application provides: a method for producing human induced pluripotent stem (iPS) cells comprising an exogenous chromosome having a DNA of interest using the MMCT method; and a method for expressing the exogenous gene in the human iPS cells prepared by the method for producing human iPS cells, or in undifferentiated or differentiated cells derived from the human iPS cells induced to differentiate from the human iPS cells.Type: ApplicationFiled: October 10, 2019Publication date: April 29, 2021Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, Trans Chromosomics, Inc.Inventors: Yasuhiro KAZUKI, Narumi UNO, Mitsuo OSHIMURA
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Publication number: 20210095311Abstract: A mouse artificial chromosome vector is stable in rodent cells, tissues, and/or individuals, specifically a mouse artificial chromosome vector derived from a mouse chromosome selected from mouse chromosome 10 and mouse chromosome 16. A cell or a non-human animal may include the vector. The vector may be used for producing proteins and human antibodies.Type: ApplicationFiled: March 15, 2019Publication date: April 1, 2021Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, Trans Chromosomics, Inc.Inventors: Yasuhiro KAZUKI, Mitsuo OSHIMURA, Satoshi ABE
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Publication number: 20200332315Abstract: This application provides: a nucleic acid construct characterized by comprising, in a mammalian artificial chromosome vector, a DNA sequence of interest, a matrix attachment region, and a replication origin, and by using for high expression of the DNA; a mammalian cell comprising the nucleic acid construct; and a method for high production of a protein encoded by the DNA of interest in the mammalian cell.Type: ApplicationFiled: November 2, 2018Publication date: October 22, 2020Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, Trans Chromosomics, Inc.Inventors: Yasuhiro KAZUKI, Takahito OHIRA, Narumi UNO, Hiroyuki KUGOH, Mitsuo OSHIMURA
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Publication number: 20200029539Abstract: In this application, the provided are: a Down syndrome rat model characterized in that a rat gene homologous to at least one gene present on a human chromosome 21 or fragment thereof is a trisomy and is transmittable to progeny; or a Down syndrome rat model characterized in that it comprises a human chromosome 21 or fragment thereof, or an exogenous rat chromosome or fragment thereof on which a rat gene homologous to the human chromosome 21 or fragment thereof is present, wherein at least one gene on the human chromosome 21 or fragment thereof or on the exogenous rat chromosome or fragment thereof is added to endogenous rat genes homologous to the at least gene so as to become a trisomy and to be transmittable to progeny: and a method for producing the Down syndrome rat model.Type: ApplicationFiled: September 28, 2017Publication date: January 30, 2020Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, TRANS CHROMOSOMICS, INC.Inventors: Yasuhiro KAZUKI, Mitsuo OSHIMURA
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Publication number: 20190254264Abstract: This application provides: a non-human animal that comprises a mouse artificial chromosome comprising a human antibody heavy chain gene or gene locus, a human antibody light chain ? gene or gene locus, and/or a human antibody light chain ? gene or gene locus, and in which endogenous antibody genes or gene loci corresponding to at least 2 human antibody genes or gene loci have been knocked out, wherein the animal can be stably retained through generations and can produce human antibodies; a method for producing the non-human animal; and a method for producing human antibodies using the non-human animal.Type: ApplicationFiled: October 31, 2017Publication date: August 22, 2019Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, TRANS CHROMOSOMICS, INC.Inventors: Yasuhiro KAZUKI, Satoshi ABE, Mitsuo OSHIMURA
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Patent number: 9775331Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.Type: GrantFiled: December 18, 2014Date of Patent: October 3, 2017Assignees: National University Corporation Tottori University, Trans Chromosomics, Inc.Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
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Patent number: 9420769Abstract: A chimeric non-human animal having an in vivo human hepatocyte population, wherein the effects of non-human animal cells on drug metabolism are suppressed or deleted is provided. A method for producing a chimeric non-human animal that lacks a drug-metabolizing system or has a suppressed drug-metabolizing system and is provided with a drug-metabolizing system driven by human hepatocytes, is provided. The method comprises transplanting human hepatocytes into a non-human animal characterized by (i) being immunodeficient, (ii) having liver damage, and (iii) lacking the functions of an endogenous Cyp3a gene.Type: GrantFiled: October 12, 2012Date of Patent: August 23, 2016Assignees: PHOENIXBIO CO., LTD., NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITYInventors: Mitsuo Oshimura, Yasuhiro Kazuki, Chise Mukaidani, Takashi Shimada, Masakazu Kakuni, Satoko Hamamura, Hidetaka Kamimura, Akio Kawamura, Naoyuki Nakada, Masato Ohbuchi, Kota Kato
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Publication number: 20150284812Abstract: It is an object of the present invention to evaluate the induction of a drug-metabolizing enzyme by a test substance more accurately than ever before, by simple operations, using a vector in which a reporter gene is connected downstream of the expression control region of a drug-metabolizing enzyme gene and a cell into which the aforementioned vector is introduced. The present invention provides a vector for producing a cell that is used for evaluation of the induction of a drug-metabolizing enzyme by a test substance and the cytotoxicity of the test substance, the vector comprising an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene, wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene.Type: ApplicationFiled: October 21, 2013Publication date: October 8, 2015Applicant: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITYInventors: Masako Tada, Mitsuo Oshimura, Fumihiko Kawamura, Saori Tsuji
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Publication number: 20150096063Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.Type: ApplicationFiled: December 18, 2014Publication date: April 2, 2015Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, CHROMOCENTER INC.Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
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Patent number: 8951789Abstract: This invention relates to a mammalian artificial chromosome vector, which retains a human chromosome 7 fragment comprising human cytochrome P450 genes and is transmittable to progeny, wherein the human chromosome 7 fragment retains a region of approximately 1 MbĀ±500 Kb in size comprising at least a human CYP3A gene cluster, which region is located between chromosome markers AC004922 and AC073842, and to a non-human mammalian animal retaining the vector.Type: GrantFiled: October 14, 2008Date of Patent: February 10, 2015Assignees: National University Corporation Tottori University, Chromocenter Inc.Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Takashi Matsuoka, Kazuma Tomizuka, Takeshi Oshima
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Patent number: 8940533Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.Type: GrantFiled: January 6, 2011Date of Patent: January 27, 2015Assignees: National University Corporation Tottori University, Chromocenter Inc.Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
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Publication number: 20140241991Abstract: A chimeric non-human animal having an in vivo human hepatocyte population, wherein the effects of non-human animal cells on drug metabolism are suppressed or deleted is provided. A method for producing a chimeric non-human animal that lacks a drug-metabolizing system or has a suppressed drug-metabolizing system and is provided with a drug-metabolizing system driven by human hepatocytes, is provided. The method comprises transplanting human hepatocytes into a non-human animal characterized by (i) being immunodeficient, (ii) having liver damage, and (iii) lacking the functions of an endogenous Cyp3a gene.Type: ApplicationFiled: October 12, 2012Publication date: August 28, 2014Applicants: PHOENIXBIO CO., LTD., NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITYInventors: Mitsuo Oshimura, Yasuhiro Kazuki, Chise Mukaidani, Takashi Shimada, Masakazu Kakuni, Satoko Hamamura, Hidetaka Kamimura, Akio Kawamura, Naoyuki Nakada, Masato Ohbuchi, Kota Kato
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Patent number: 8809045Abstract: This invention relates to a human artificial chromosome (HAC) vector carrying a human chromosome-derived centromere, a subtelomere sequence, and a telomere sequence, to a human cell medicine or human cells comprising the HAC vector, to methods for preparing the HAC vector and human cells, and to methods for producing a therapeutic protein using the HAC vector.Type: GrantFiled: July 6, 2007Date of Patent: August 19, 2014Assignee: Kyowa Hakko Kirin Co., LtdInventors: Minoru Kakeda, Kazuma Tomizuka, Mitsuo Oshimura, Yasuhiro Kazuki
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Patent number: 8703482Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.Type: GrantFiled: October 7, 2010Date of Patent: April 22, 2014Assignee: Kyowa Hakko Kirin Co., LtdInventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
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Publication number: 20120272342Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.Type: ApplicationFiled: January 6, 2011Publication date: October 25, 2012Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
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Publication number: 20120093785Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.Type: ApplicationFiled: October 7, 2010Publication date: April 19, 2012Inventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
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Patent number: 8124406Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.Type: GrantFiled: December 28, 2010Date of Patent: February 28, 2012Assignee: Kyowa Hakko Kirin Co., LtdInventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa