Patents by Inventor Mitsuo Oshimura

Mitsuo Oshimura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200332315
    Abstract: This application provides: a nucleic acid construct characterized by comprising, in a mammalian artificial chromosome vector, a DNA sequence of interest, a matrix attachment region, and a replication origin, and by using for high expression of the DNA; a mammalian cell comprising the nucleic acid construct; and a method for high production of a protein encoded by the DNA of interest in the mammalian cell.
    Type: Application
    Filed: November 2, 2018
    Publication date: October 22, 2020
    Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, Trans Chromosomics, Inc.
    Inventors: Yasuhiro KAZUKI, Takahito OHIRA, Narumi UNO, Hiroyuki KUGOH, Mitsuo OSHIMURA
  • Publication number: 20200029539
    Abstract: In this application, the provided are: a Down syndrome rat model characterized in that a rat gene homologous to at least one gene present on a human chromosome 21 or fragment thereof is a trisomy and is transmittable to progeny; or a Down syndrome rat model characterized in that it comprises a human chromosome 21 or fragment thereof, or an exogenous rat chromosome or fragment thereof on which a rat gene homologous to the human chromosome 21 or fragment thereof is present, wherein at least one gene on the human chromosome 21 or fragment thereof or on the exogenous rat chromosome or fragment thereof is added to endogenous rat genes homologous to the at least gene so as to become a trisomy and to be transmittable to progeny: and a method for producing the Down syndrome rat model.
    Type: Application
    Filed: September 28, 2017
    Publication date: January 30, 2020
    Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, TRANS CHROMOSOMICS, INC.
    Inventors: Yasuhiro KAZUKI, Mitsuo OSHIMURA
  • Publication number: 20190254264
    Abstract: This application provides: a non-human animal that comprises a mouse artificial chromosome comprising a human antibody heavy chain gene or gene locus, a human antibody light chain ? gene or gene locus, and/or a human antibody light chain ? gene or gene locus, and in which endogenous antibody genes or gene loci corresponding to at least 2 human antibody genes or gene loci have been knocked out, wherein the animal can be stably retained through generations and can produce human antibodies; a method for producing the non-human animal; and a method for producing human antibodies using the non-human animal.
    Type: Application
    Filed: October 31, 2017
    Publication date: August 22, 2019
    Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, TRANS CHROMOSOMICS, INC.
    Inventors: Yasuhiro KAZUKI, Satoshi ABE, Mitsuo OSHIMURA
  • Patent number: 9775331
    Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: October 3, 2017
    Assignees: National University Corporation Tottori University, Trans Chromosomics, Inc.
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
  • Patent number: 9420769
    Abstract: A chimeric non-human animal having an in vivo human hepatocyte population, wherein the effects of non-human animal cells on drug metabolism are suppressed or deleted is provided. A method for producing a chimeric non-human animal that lacks a drug-metabolizing system or has a suppressed drug-metabolizing system and is provided with a drug-metabolizing system driven by human hepatocytes, is provided. The method comprises transplanting human hepatocytes into a non-human animal characterized by (i) being immunodeficient, (ii) having liver damage, and (iii) lacking the functions of an endogenous Cyp3a gene.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: August 23, 2016
    Assignees: PHOENIXBIO CO., LTD., NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Chise Mukaidani, Takashi Shimada, Masakazu Kakuni, Satoko Hamamura, Hidetaka Kamimura, Akio Kawamura, Naoyuki Nakada, Masato Ohbuchi, Kota Kato
  • Publication number: 20150284812
    Abstract: It is an object of the present invention to evaluate the induction of a drug-metabolizing enzyme by a test substance more accurately than ever before, by simple operations, using a vector in which a reporter gene is connected downstream of the expression control region of a drug-metabolizing enzyme gene and a cell into which the aforementioned vector is introduced. The present invention provides a vector for producing a cell that is used for evaluation of the induction of a drug-metabolizing enzyme by a test substance and the cytotoxicity of the test substance, the vector comprising an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene, wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene.
    Type: Application
    Filed: October 21, 2013
    Publication date: October 8, 2015
    Applicant: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY
    Inventors: Masako Tada, Mitsuo Oshimura, Fumihiko Kawamura, Saori Tsuji
  • Publication number: 20150096063
    Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
    Type: Application
    Filed: December 18, 2014
    Publication date: April 2, 2015
    Applicants: NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY, CHROMOCENTER INC.
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
  • Patent number: 8951789
    Abstract: This invention relates to a mammalian artificial chromosome vector, which retains a human chromosome 7 fragment comprising human cytochrome P450 genes and is transmittable to progeny, wherein the human chromosome 7 fragment retains a region of approximately 1 Mb±500 Kb in size comprising at least a human CYP3A gene cluster, which region is located between chromosome markers AC004922 and AC073842, and to a non-human mammalian animal retaining the vector.
    Type: Grant
    Filed: October 14, 2008
    Date of Patent: February 10, 2015
    Assignees: National University Corporation Tottori University, Chromocenter Inc.
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Takashi Matsuoka, Kazuma Tomizuka, Takeshi Oshima
  • Patent number: 8940533
    Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
    Type: Grant
    Filed: January 6, 2011
    Date of Patent: January 27, 2015
    Assignees: National University Corporation Tottori University, Chromocenter Inc.
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
  • Publication number: 20140241991
    Abstract: A chimeric non-human animal having an in vivo human hepatocyte population, wherein the effects of non-human animal cells on drug metabolism are suppressed or deleted is provided. A method for producing a chimeric non-human animal that lacks a drug-metabolizing system or has a suppressed drug-metabolizing system and is provided with a drug-metabolizing system driven by human hepatocytes, is provided. The method comprises transplanting human hepatocytes into a non-human animal characterized by (i) being immunodeficient, (ii) having liver damage, and (iii) lacking the functions of an endogenous Cyp3a gene.
    Type: Application
    Filed: October 12, 2012
    Publication date: August 28, 2014
    Applicants: PHOENIXBIO CO., LTD., NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Chise Mukaidani, Takashi Shimada, Masakazu Kakuni, Satoko Hamamura, Hidetaka Kamimura, Akio Kawamura, Naoyuki Nakada, Masato Ohbuchi, Kota Kato
  • Patent number: 8809045
    Abstract: This invention relates to a human artificial chromosome (HAC) vector carrying a human chromosome-derived centromere, a subtelomere sequence, and a telomere sequence, to a human cell medicine or human cells comprising the HAC vector, to methods for preparing the HAC vector and human cells, and to methods for producing a therapeutic protein using the HAC vector.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: August 19, 2014
    Assignee: Kyowa Hakko Kirin Co., Ltd
    Inventors: Minoru Kakeda, Kazuma Tomizuka, Mitsuo Oshimura, Yasuhiro Kazuki
  • Patent number: 8703482
    Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.
    Type: Grant
    Filed: October 7, 2010
    Date of Patent: April 22, 2014
    Assignee: Kyowa Hakko Kirin Co., Ltd
    Inventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
  • Publication number: 20120272342
    Abstract: Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
    Type: Application
    Filed: January 6, 2011
    Publication date: October 25, 2012
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
  • Publication number: 20120093785
    Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.
    Type: Application
    Filed: October 7, 2010
    Publication date: April 19, 2012
    Inventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
  • Patent number: 8124406
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: February 28, 2012
    Assignee: Kyowa Hakko Kirin Co., Ltd
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Patent number: 8110671
    Abstract: The specification relates to a method for producing a chimeric non-human animal, which comprises preparing a microcell containing a foreign chromosome(s) or a fragment(s) thereof and transferring the foreign chromosome(s) or fragment(s) thereof into a pluripotent cell by fusion with the microcell; a chimeric non-human animal which can be produced by the above method and its progeny; tissues and cells derived therefrom; and a method for using the same. Further, a pluripotent cell containing a foreign chromosome(s) or a fragment(s) thereof, a method for producing the same, and a method for using the same are also provided. Moreover, a pluripotent cell in which at least two endogenous genes are disrupted, and a method for producing the same by homologous recombination are provided.
    Type: Grant
    Filed: May 8, 2007
    Date of Patent: February 7, 2012
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida
  • Publication number: 20110151518
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Application
    Filed: December 28, 2010
    Publication date: June 23, 2011
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Publication number: 20110023138
    Abstract: This invention relates to a mammalian artificial chromosome vector, which retains a human chromosome 7 fragment comprising human cytochrome P450 genes and is transmittable to progeny, wherein the human chromosome 7 fragment retains a region of approximately 1 Mb±500 Kb in size comprising at least a human CYP3A gene cluster, which region is located between chromosome markers AC004922 and AC073842, and to a non-human mammalian animal retaining the vector.
    Type: Application
    Filed: October 14, 2008
    Publication date: January 27, 2011
    Applicants: National University Corporation Tottori University, Chromocenter Inc.
    Inventors: Mitsuo Oshimura, Yasuhiro Kazuki, Takashi Matsuoka, Kazuma Tomizuka, Takeshi Oshima
  • Patent number: 7868223
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Grant
    Filed: May 7, 2008
    Date of Patent: January 11, 2011
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Publication number: 20100011454
    Abstract: This invention relates to a human artificial chromosome (HAC) vector carrying a human chromosome-derived centromere, a subtelomere sequence, and a telomere sequence, to a human cell medicine or human cells comprising the HAC vector, to methods for preparing the HAC vector and human cells, and to methods for producing a therapeutic protein using the HAC vector.
    Type: Application
    Filed: July 6, 2007
    Publication date: January 14, 2010
    Inventors: Minoru Kakeda, Kazuma Tomizuka, Mitsuo Oshimura, Yasuhiro Kazuki