Abstract: The present invention relates to a method of producing a protein for which two or more characteristics are optimized simultaneously. More specifically, the present invention relates to a method of producing a protein for which two or more characteristics are optimized, the method comprising: 1) providing a library comprising mutants from random mutation of a target protein; 2) determining respective characteristic values that indicate the two or more characteristics of some of the mutants in the library, and scoring the two or more characteristic values as one value per mutant by normalizing and integrating the characteristic values; 3) conducting machine learning by using the score values and ranking the library; and 4) selecting a protein for which two or more characteristics are optimized, based on the ranking results, wherein the two or more characteristic values are numerical values based on different measurement data related to respective different characteristics.

Abstract: An object of the present invention is to provide a bacterium of the genus Bifidobacterium exerting an anti-tumor effect against solid cancers sustainably and specifically. Produced is a bacterium of the genus Bifidobacterium expressing a first polypeptide comprising a light-chain variable region (VL) that binds to human CD3 and a heavy-chain variable region (VH) that binds to a human tumor cell surface antigen; and a second polypeptide comprising VL that binds to a human tumor cell surface antigen and VH that binds to human CD3 and secreting a diabody-type bispecific antibody composed of the first polypeptide and the second polypeptide.

Abstract: To provide bispecific antibodies and highly functional bispecific antibodies, which are produced by using a new anti-human EGF receptor 1 (Her 1) antibody different from the antibody 528. A bispecific antibody, comprising a variable region of the light chain (2L: SEQ ID NO:2) and a variable region of the heavy chain (2H: SEQ ID NO:4) of an anti-human EGF receptor 1 antibody 225, and a humanized variable region of the light chain (OL: SEQ ID NO:6) and a humanized variable region of the heavy chain (OH: SEQ ID NO:8) of an anti-CD3 antibody OKT, and the like.

Abstract: To provide a high-functional bispecific antibody, especially LH-type bispecific antibody that has further improved its function while maintaining the excellent cytotoxicity such as anti-tumor activity. A bispecific antibody for human EGF receptor 1 and CD3, comprising a variable region comprising a humanized variable region of the light chain (5L: SEQ ID NO:2) and a humanized variable region of the heavy chain (5H: SEQ ID NO:4) of an anti-human EGF receptor 1 antibody 528, and a humanized variable region of the light chain (OL: SEQ ID NO:6) and of a humanized variable region of the heavy chain (OH: SEQ ID NO:8) of an anti-CD3 antibody OKT; a hinge region; and an Fc region, characterized by that the Fc region is originated from human IgG2 sub-class.

Abstract: Provided are a novel diabody type bispecific antibody, the function of which as a bispecific antibody is improved to provide a higher additional value, such as cost saving caused by a reduction in dose, to a drug; and a method for producing the same. A humanized diabody type bispecific antibody (LH-diabody type bispecific antibody) characterized in that an L-chain is located in the N-terminal side in each polypeptide (LH type); a humanized high-functional bispecific antibody which contains said LH diabody type bispecific antibody; a nucleic acid molecule encoding both of two kinds of single-stranded polypeptides constituting said bispecific antibody; and a method for producing said antibody which comprises culturing a host cell having been transformed by an expression vector containing said nucleic acid molecule.

Abstract: The purpose of the present invention is to provide fragments of humanized anti-EGFR antibody substituted-lysine light-chain or heavy-chain variable regions, and single-chain antibodies, etc, comprising the same, having sufficient binding activity (affinity) with target cells, and having the ability to undergo various site-specific and uniform chemical modifications. The present invention pertains to a humanized variable region on the light-chain or heavy-chain of antibody 528 against human epidermal cell growth factor receptor 1 (Her-1), said variable region comprising the amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4, wherein fragments of humanized anti-EGFR antibody lysine-substituted light-chain variable regions are formed by substituting a different amino acid for all of the lysine residues, or all of the lysine residues except for the lysine residue(s) of one specified moiety.

Abstract: The purpose of the present invention is to provide fragments of humanized anti-EGFR antibody substituted-lysine light-chain or heavy-chain variable regions, and single-chain antibodies, etc, comprising the same, having sufficient binding activity (affinity) with target cells, and having the ability to undergo various site-specific and uniform chemical modifications. The present invention pertains to a humanized variable region on the light-chain or heavy-chain of antibody 528 against human epidermal cell growth factor receptor 1 (Her-1), said variable region comprising the amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4, wherein fragments of humanized anti-EGFR antibody lysine-substituted light-chain variable regions are formed by substituting a different amino acid for all of the lysine residues, or all of the lysine residues except for the lysine residue(s) of one specified moiety.

Abstract: Provided are a novel diabody type bispecific antibody, the function of which as a bispecific antibody is improved to provide a higher additional value, such as cost saving caused by a reduction in dose, to a drug; and a method for producing the same. A humanized diabody type bispecific antibody (LH-diabody type bispecific antibody) characterized in that an L-chain is located in the N-terminal side in each polypeptide (LH type); a humanized high-functional bispecific antibody which contains said LH diabody type bispecific antibody; a nucleic acid molecule encoding both of two kinds of single-stranded polypeptides constituting said bispecific antibody; and a method for producing said antibody which comprises culturing a host cell having been transformed by an expression vector containing said nucleic acid molecule.

Abstract: [Problem] Disclosed is an antibody that exhibits excellent cytotoxicity and cell growth inhibition and that is based on an anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528). Further disclosed is a method for producing same, and the like. [Solution] The mutant of an H chain humanized variable region (5H) or an L chain humanized variable region (5L) of the anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528) is the aforementioned antibody characterized by having one to a plurality (for example: 1 to 5, or 1 to 3) of amino acid mutations within CDR2. Further disclosed are antibody molecules containing said region, a nucleic acid molecule coding for these polypeptides, a method for producing said antibody molecules, and the like.

Abstract: The purpose of the present invention is to provide fragments of humanized anti-EGFR antibody substituted-lysine light-chain or heavy-chain variable regions, and single-chain antibodies, etc, comprising the same, having sufficient binding activity (affinity) with target cells, and having the ability to undergo various site-specific and uniform chemical modifications. The present invention pertains to a humanized variable region on the light-chain or heavy-chain of antibody 528 against human epidermal cell growth factor receptor 1 (Her-1), said variable region comprising the amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4, wherein fragments of humanized anti-EGFR antibody lysine-substituted light-chain variable regions are formed by substituting a different amino acid for all of the lysine residues, or all of the lysine residues except for the lysine residue(s) of one specified moiety.

Abstract: [Problem] Disclosed is an antibody that exhibits excellent cytotoxicity and cell growth inhibition and that is based on an anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528). Further disclosed is a method for producing same, and the like. [Solution] The mutant of an H chain humanized variable region (5H) or an L chain humanized variable region (5L) of the anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528) is the aforementioned antibody characterized by having one to a plurality (for example: 1 to 5, or 1 to 3) of amino acid mutations within CDR2. Further disclosed are antibody molecules containing said region, a nucleic acid molecule coding for these polypeptides, a method for producing said antibody molecules, and the like.

Abstract: Provided are a novel diabody type bispecific antibody, the function of which as a bispecific antibody is improved to provide a higher additional value, such as cost saving caused by a reduction in dose, to a drug; and a method for producing the same. A humanized diabody type bispecific antibody (LH-diabody type bispecific antibody) characterized in that an L-chain is located in the N-terminal side in each polypeptide (LH type); a humanized high-functional bispecific antibody which contains said LH diabody type bispecific antibody; a nucleic acid molecule encoding both of two kinds of single-stranded polypeptides constituting said bispecific antibody; and a method for producing said antibody which comprises culturing a host cell having been transformed by an expression vector containing said nucleic acid molecule.

Abstract: The present invention relates to a zinc oxide-binding antibody having high stability and binding activity, and high-throughput sensing technology using the antibody, such as biosensors. Specifically, the present invention relates to a peptide-grafted antibody that contains a zinc oxide-recognizing peptide in the CDR H-1 region of a camel antibody, and a solid support (e.g., a biosensor and a protein chip) containing a zinc oxide layer on which the antibody has been immobilized.