Abstract: The present invention is to provide a cell chip and a three-dimensional tissue chip and a production method therefor such that even when a highly viscous cell-containing solution is a material, the highly safe and reproducible cell chip or three-dimensional tissue chip with a desired application volume can be produced within a short time and in a large quantity so as to have a desired cell density and exhibit high cell viability.

Abstract: This cellular tissue production method can produce a cellular tissue with high density and high shape retainability while the cell viability is maintained, and includes: a first application step of applying a first application liquid to an application target; and a second application step of applying and layering a second application liquid onto the first application liquid applied in the first application step, wherein the second application step includes subjecting an interface between the first application liquid and the second application liquid to gelation while the first application liquid is kept fluid.

Abstract: An application apparatus includes: an application needle that applies, to a target, an application material having its viscosity changing under shear; a drive unit that moves the application needle up and down; and a controller that controls the drive unit to move the application needle such that shear is applied to the application material at a shear speed depending on a type of the application material and depending on a target application amount or a target application diameter.

Abstract: The present invention is to improve dynamic characteristics while maintaining temperature responsiveness in a cell culture support containing hydroxyalkyl chitosan, and to provide a cell culture support having temperature responsiveness, which contains temperature-responsive hydroxyalkyl chitosan and a water-soluble polymer selected from polyethylene glycol, derivatives thereof, hyaluronic acids, alginic acids and salts thereof.

Abstract: The present invention provides a medium for inducing the differentiation of stem cells into mesodermal cells, the medium comprising a ROCK inhibitor, a bone morphogenetic protein (BMP), a fibroblast growth factor (FGF), and activin. The present invention also provides a method for producing mesodermal cells from stem cells using the medium, and method for producing cardiac progenitor cells or myocardiocytes from stem cells using the medium. The present invention furthermore provides a composition for assisting the induction of differentiation of stem cells into mesodermal cells, the composition comprising a ROCK inhibitor, a BMP, an FGF, and activin. The present invention additionally provides cell groups obtained by the above methods.

Abstract: The present disclosure relates to: an artificial peritoneal tissue comprising a cellular tissue and a mesothelial cell layer that covers the surface of the cellular tissue, wherein the cellular tissue comprises a fibroblast, an extracellular matrix, and a vascular endothelial cell and/or a lymphatic endothelial cell each capable of forming a lumen; and a method for producing the artificial peritoneal tissue.

Abstract: An artificial tissue containing pancreatic islet cells, fibroblasts and/or cells capable of differentiating, extracellular matrix, and vascular endothelial cells, in which the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute a three-dimensional tissue structure in which a vascular network structure has been formed, and in which the three-dimensional tissue structure contains pancreatic islets constituted by aggregating ten or more of the pancreatic islet cells.

Abstract: The present invention is to provide a cell chip and a three-dimensional tissue chip and a production method therefor such that even when a highly viscous cell-containing solution is a material, the highly safe and reproducible cell chip or three-dimensional tissue chip with a desired application volume can be produced within a short time and in a large quantity so as to have a desired cell density and exhibit high cell viability.

Abstract: A polylactide derivative according to the present invention is expressed by the following general formula (1) or general formula (2): In general formula (1), one of X1-X5 is an aldehyde group, one of the other four is an alkoxy group, and the other three are hydrogen atoms. In general formula (2), one of R1-R3 is selected from a chlorine atom, fluorine atom, aldehyde group, alkoxy group, alkyl group and ester, while the other two are selected from the chlorine atom, fluorine atom, aldehyde group, alkoxy group, alkyl group, ester, and hydrogen atom.

Abstract: Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells (1), each of which is obtained by covering the surface of a cell (3) with a coating film (2) containing an extracellular matrix component; forming a dermis tissue layer (7), in which the coated cells (1) are laminated, by culturing the coated cells (1); and forming an epidermis layer (12) by arranging epidermis cells (8) on the dermis tissue layer (7).

Abstract: A polylactide derivative according to the present invention is expressed by the following general formula (1) or general formula (2): In general formula (1), one of X1-X5 is an aldehyde group, one of the other four is an alkoxy group, and the other three are hydrogen atoms. In general formula (2), one of R1-R3 is selected from a chlorine atom, fluorine atom, aldehyde group, alkoxy group, alkyl group and ester, while the other two are selected from the chlorine atom, fluorine atom, aldehyde group, alkoxy group, alkyl group, ester, and hydrogen atom.

Abstract: An artificial tissue containing pancreatic islet cells, fibroblasts and/or cells capable of differentiating, extracellular matrix, and vascular endothelial cells, in which the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute a three-dimensional tissue structure in which a vascular network structure has been formed, and in which the three-dimensional tissue structure contains pancreatic islets constituted by aggregating ten or more of the pancreatic islet cells.

Abstract: The present disclosure relates to: an artificial peritoneal tissue comprising a cellular tissue and a mesothelial cell layer that covers the surface of the cellular tissue, wherein the cellular tissue comprises a fibroblast, an extracellular matrix, and a vascular endothelial cell and/or a lymphatic endothelial cell each capable of forming a lumen; and a method for producing the artificial peritoneal tissue.

Abstract: Provided are an elastic three-dimensional tissue and a method by which the tissue can be produced. The elastic three-dimensional tissue includes smooth muscle cells and an extracellular matrix component, with the smooth muscle cells being layered with the extracellular matrix component interposed therebetween. Furthermore, the production method for a three-dimensional tissue includes layering smooth muscle cells with an extracellular matrix component interposed therebetween, wherein the smooth muscle cells are those directed towards a differentiated type from an undifferentiated type.

Abstract: The present invention is to provide a material that is capable of selectively adsorbing organic fluoro-compounds such as perfluorooctane sulfonic acid, allows the adsorbed organic fluoro-compounds to be recovered, and is reusable as an adsorbent, specifically to provide a polymer in which cyclodextrin is supported on the surface of a water-insoluble polymer, and an adsorbent containing the same, and a method of use of the same as a selective adsorbent of, in particular, an organic fluoro-compound.

Abstract: The present invention is to provide a material that is capable of selectively adsorbing organic fluoro-compounds such as perfluorooctane sulfonic acid, allows the adsorbed organic fluoro-compounds to be recovered, and is reusable as an adsorbent, specifically to provide a polymer in which cyclodextrin is supported on the surface of a water-insoluble polymer, and an adsorbent containing the same, and a method of use of the same as a selective adsorbent of, in particular, an organic fluoro-compound.

Abstract: Provided is a novel method capable of manufacturing an artificial skin model including dendritic cells. A method for manufacturing an artificial skin model includes the following: forming a dermal tissue layer by culturing coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component, so that the coated cells are layered; forming a basal layer including type IV collagen on the dermal tissue layer by bringing type IV collagen into contact with the dermal tissue layer; and forming an epidermal layer by arranging epidermal cells on the basal layer. At least one of the dermal tissue layer and the epidermal layer includes dendritic cells.

Abstract: Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 ?L or more of the culture medium per 1×104 cells.

Abstract: A polyion complex (PIC) or a PIC nanoparticle that may be easily prepared, and that is finally disappeared in vivo due to its suitable biodegradability while exhibiting high stability in vivo, an immunotherapy agent comprising the PIC nanoparticle to which various antigen proteins or peptides may be easily conjugated or incorporated and/or which may be easily mixed with the antigen proteins or peptides, as well as a process for preparing thereof are provided. Specifically, a polyion complex (PIC) comprising a hydrophobized poly(acidic amino acid) and a basic polypeptide, a nanoparticle thereof having a particle shape, an immunotherapy agent comprising the PIC nanoparticle, as well as a process for preparing the PIC, comprising steps of introducing a hydrophobic amino acid to a poly(acidic amino acid) to prepare a hydrophobized poly(acidic amino acid), and dissolving the hydrophobized poly(acidic amino acid) prepared to a buffer, and it is mixed with a basic polypeptide dissolved in a buffer.

Abstract: A hydrophilic material having a hydrophilic nature and being biocompatible and processable into nanoparticles wherein a hydrophilic monomer such as threitol or a hydrophilic polymer such as polyethylene glycol is attached to the ?position of an ?,?-unsaturated carboxyl group of a (co)polymer comprising an aromatic compound such as 4-hydroxycinnamic acid in which an aromatic ring is substituted by the ?,?-unsaturated carboxyl group and one or more hydroxyl groups. In particular, a hydrophilic material having a nanoparticle size which is highly dispersible in water, can well migrate into the blood and is useful as a drug carrier, a medical material and a sustained drug release material.