Abstract: A concentration of cholesterol ester or free cholesterol can be calculated using the concentrations of total cholesterol and triglyceride in lipoproteins contained in a sample. Further, a concentration of lipoprotein particles in a fraction can be calculated using the lipoprotein particle size in the fraction. The concentrations of total cholesterol and triglyceride contained in a subject sample are detected. Using the concentrations of total cholesterol and triglyceride thus detected, the concentration of cholesterol ester or free cholesterol is calculated. Further, using the concentration of cholesterol ester or free cholesterol thus calculated, the concentration of lipoprotein particles in a fraction can be calculated.

Abstract: A concentration of cholesterol ester or free cholesterol can be calculated using the concentrations of total cholesterol and triglyceride in lipoproteins contained in a sample. Further, a concentration of lipoprotein particles in a fraction can be calculated using the lipoprotein particle size in the fraction. The concentrations of total cholesterol and triglyceride contained in a subject sample are detected. Using the concentrations of total cholesterol and triglyceride thus detected, the concentration of cholesterol ester or free cholesterol is calculated. Further, using the concentration of cholesterol ester or free cholesterol thus calculated, the concentration of lipoprotein particles in a fraction can be calculated.

Abstract: A method of analysis of lipoproteins according to the present invention comprises the steps of: separating a plurality of classes of lipoproteins contained in a subject sample by liquid chromatography and then detecting signals derived from components included in the separated lipoproteins; and assuming that the lipoproteins are constituted of subclasses estimated from component peaks comprising anchor peaks and extra essential peaks and then calculating an approximated curve corresponding to summation of the component peaks using the above described detected signals.

Abstract: A method of analysis of lipoproteins according to the present invention comprises the steps of: separating a plurality of classes of lipoproteins contained in a subject sample by liquid chromatography and then detecting signals derived from components included in the separated lipoproteins; and assuming that the lipoproteins are constituted of subclasses estimated from component peaks comprising anchor peaks and extra essential peaks and then calculating an approximated curve corresponding to summation of the component peaks using the above described detected signals.

Abstract: On a chromatogram wherein peaks corresponding to a plurality of classes of lipoproteins appear in a mixed form, components contained in the lipoproteins are quantitatively determined by class. The present invention comprises a first step of separating a plurality of classes of lipoproteins contained in a sample through liquid chromatography and assaying the components contained in the separated lipoproteins and a second step of dividing the chromatogram obtained in the above described first step by lipoprotein class and determining quantitatively the components contained in the lipoproteins by class.