Patents by Inventor Mitsuyoshi Morii

Mitsuyoshi Morii has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for purifying a crude tissue plasminogen activator preparation
    Patent number: 5158882
    Abstract: Separation of tPA and undesired proteins is securely and effectivity accomplished by using a cation-exchanger in the two step procedure for selective elution of undesired proteins from the cation-exchanger on which tPA and undesired proteins are adsorbed: undesired proteins having the pI equivalent to or lower than that of tPA was eluted out in the first step and then undesired proteins having the pI equivalent to or higher than were eluted in the second step.
    Type: Grant
    Filed: February 28, 1992
    Date of Patent: October 27, 1992
    Assignee: Mitsui Toatsu Chemical Incorporated
    Inventors: Mitsuyoshi Morii, Nobuhiro Kawashima, Kunizo Mori, Masaharu Ohoka
  • Purificaiton procedure of tPA from crude preparations
    Patent number: 5110736
    Abstract: Crude tPA containing, as an impurity, a protein which is reactive with an anti-human tPA antibody and has a molecular weight of 110,000.+-.20,000 daltons is purified by bringing the crude tPA into close contact with an affinity reagent containing an immobilized Kunitz inhibitor which is produced in seeds of Erythrina latissima and other Erythrina plants and acts as an inhibitor on trypsin, plasmin and tPA but does not act on urokinase, so that tPA is collected selectively.
    Type: Grant
    Filed: October 24, 1990
    Date of Patent: May 5, 1992
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Mitsuyoshi Morii, Masaharu Ohoka, Nobuhiro Kawashima, Noriko Morii, Toshihiko Suzuki
  • Process of purifying tPA
    Patent number: 4985362
    Abstract: Tissue plasminogen activator (tPA) species having a molecular weight of about 70,000 daltons is isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with a cation exchanger in advance and separating the desired tPA species selectively from the cation exchanger by means of the salt gradient elution method.
    Type: Grant
    Filed: September 17, 1987
    Date of Patent: January 15, 1991
    Assignee: Mitsui Toatsu Chemicals Incorporated
    Inventors: Mitsuyoshi Morii, Masaharu Ohoka, Toshihiko Suzuki, Katsuyuki Suzuki, Nobuhiro Kawashima, Noriko Morii, Kunizou Mori
  • Process for separating single-chain TPA and double-chain from each other
    Patent number: 4978620
    Abstract: The distinction of tissue plasminogen activator, especially, the separation of its single-chain species and double-chain species is effected by using the Erythrina trypsin inhibitor which occurs in seeds of a plant of the Erythrina species.
    Type: Grant
    Filed: April 7, 1988
    Date of Patent: December 18, 1990
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Mitsuyoshi Morii, Masaharu Ohoka, Toshihiko Suzuki, Katsuyuki Suzuki, Nobuhiro Kawashima, Noriko Morii, Kunizo Mori
  • Methods for purification of single-chain and double-chain tissue plasminogen activator
    Patent number: 4898825
    Abstract: A mixture containing a single-chain tissue plasminogen activator (tPA) and/or double-chain tPA is brought into close contact with a column carrying an immobilized Erythrina trypsin inhibitor as an affinity agent. Adsorbed protiens are eluted with eluents having different pHs with or without arginine or benzamidine, so that single-chain tPA is obtained in the eluent with a pH at least 4.5 and double-chain tPA is obtained in the eluent with a pH lower than 4.5.
    Type: Grant
    Filed: April 29, 1987
    Date of Patent: February 6, 1990
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Mitsuyoshi Morii, Masaharu Ohoka, Toshihiko Suzuki, Katsuyuki Suzuki, Nobuhiro Kawashima, Noriko Morii, Kunizo Mori