Abstract: Disclosed is a method for manufacturing a monoclonal antibody without using animal individuals. This method includes a step of introducing a DNA fragment comprising a gene that encodes a secretory signal, a gene that encodes a nanobody, and a gene that encodes a peptide barcode, or a vector containing the DNA fragment, into a yeast cell; and a step of collecting a polypeptide comprising the nanobody and the peptide barcode that has been expressed in the cell and secreted to the outside of the cell. According to the method, it is possible to manufacture a monoclonal nanobody more efficiently in a shorter period of time without using animal individuals.

Abstract: A method and apparatus for screening a microorganism or protein having a useful function by using a micro-chamber array. Using the screening method and screening apparatus in accordance with the present invention, a target protein having an enzymatic activity or a microorganism producing such a protein is screened from a sample serving as a screening object by using a base plate having a plurality of micro-chambers arranged in the form of an array. With this method and apparatus, a protein having an enzymatic activity or a microorganism producing such a protein that is present in a micro-chamber is detected by detecting a fluorescence produced by an enzyme reaction of the protein having an enzymatic activity and a fluorescence substrate.