Abstract: An image processing device comprises pixel group setting part that sets plurality of pixel groups including first and second pixel groups, which are not connected to each other, based on image data of first image which shows target object including linear portion; pair setting part that performs setting pair of first and second pixel groups including pixel within predetermined range from first pixel group to calculate connecting degree of second pixel group with respect to first pixel group; and calculation part that calculates connecting degree of pair, wherein predetermined range is search range set based on first distance between first and second pixel groups, or search range set on based on first angle formed between first line segment corresponding to first pixel group and second line segment corresponding to second pixel group, and calculation part calculates first distance or first angle using first distance or first angle.

Abstract: A method of analyzing neurons includes: identifying a cell region by identifying a region of a neurite or a region of a cell body of a neuron on the basis of time-series images obtained by imaging the neuron in time series; detecting aggregates by imaging, in time series, first fluorescent protein tagged to specific protein expressed in the neuron and detecting presence or absence of aggregates of the specific protein aggregated in the region of the neurite or the region of the cell body identified in the identifying the cell region on the basis of luminance of the first fluorescent protein included in the time-series images; and performing an analysis by classifying the neuron into a plurality of groups on the basis of a detection result of the detecting the aggregates and analyzing a survival state of the neuron for each of the groups.

Abstract: A method for generating a learned model that performs an image transformation disclosed in the present description includes a step of learning mapping of a source to a target using a first image group as the source and at least one phase contrast image as the target, the first image group including a first image set having microscopic images of a biological sample captured at different positions along a first optical axis, the microscopic images being other than phase contrast images.

Abstract: An image processing device comprises pixel group setting part that sets plurality of pixel groups including first and second pixel groups, which are not connected to each other, based on image data of first image which shows target object including linear portion; pair setting part that performs setting pair of first and second pixel groups including pixel within predetermined range from first pixel group to calculate connecting degree of second pixel group with respect to first pixel group; and calculation part that calculates connecting degree of pair, wherein predetermined range is search range set based on first distance between first and second pixel groups, or search range set on based on first angle formed between first line segment corresponding to first pixel group and second line segment corresponding to second pixel group, and calculation part calculates first distance or first angle using first distance or first angle.