Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic macrophages through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow macrophages to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. There are provided, in some embodiments, heat-inducible macrophages and methods of using are provided.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of probiotic cells. There are provided, in some embodiments, thermal bioswitches that allow probiotic cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. Thermally actuated probiotic cells and methods of use for the treatment of diseases or disorders are also provided.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.