Abstract: An engineered plant-derived transfer (P-)DNA was designed and constructed based on a couple of T-DNA homologous sequences in sugar beet genome. Plant transformation efficacy of the engineered P-DNA was analyzed compared to conventional T-DNA in two independent systems, stable transformation of tobacco plant and sugar beet hairy roots. The outcomes demonstrated that plant transformation is directed well by vectors carrying the engineered P-DNA with higher efficiency than the conventional binary vector in both experimental systems. This vector was further improved by adding two matrix attachment regions within P-DNA. This new vector was even more efficient in gene transfer, higher than conventional binary vector.

Abstract: An engineered plant-derived transfer (P-)DNA was designed and constructed based on a couple of T-DNA homologous sequences in sugar beet genome. Plant transformation efficacy of the engineered P-DNA was analyzed compared to conventional T-DNA in two independent systems, stable transformation of tobacco plant and sugar beet hairy roots. The outcomes demonstrated that plant transformation is directed well by vectors carrying the engineered P-DNA with higher efficiency than the conventional binary vector in both experimental systems. This vector was further improved by adding two matrix attachment regions within P-DNA. This new vector was even more efficient in gene transfer, higher than conventional binary vector.

Abstract: The various embodiments herein provide nucleic acid sequences isolated from Pseudomonas putida strain P13 encoding a family of APases including a phytase and a sugar phosphatase which are highly active at a temperature of 60° C. and at a broad range of pH and withstand the harsh conditions of food processing and digestive system of animals. The enzymes are active at a wide temperature range of 20° C. to 75° C. and at a pH of 5. The embodiments also provide a method of production of the APases. The embodiments also provide a method of isolation and cloning of the APases.

Abstract: The various embodiments herein provide nucleic acid sequences isolated from Pseudomonas putida strain P13 encoding a novel family of APases including a phytase and a sugar phosphatase which are highly active at a temperature of 60° C. and at a broad range of pH and withstand the harsh conditions of food processing and digestive system of animals. The enzymes are active at a wide temperature range of 20° C. to 75° C. and at a pH of 5. The embodiments also provide a method of production of the novel APases. The embodiments also provide a method of isolation and cloning of novel APases.