Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: A service provider provides flexible access to services using an identity provider. The service provider is associated with a custom access policy used by the identity provider to authenticate access requests associated with client devices for services of the client system. The custom access policy describes a set of access levels corresponding to variable levels of access to services of the service provider. The identity provider authenticates access requests by client devices using one or more device signals from the client devices. In some embodiments, the identity provider determines a device trust score for the client device using the one or more device signals. The identity provider provides an authentication response to the client system based on the custom access policy. The client system uses the authentication response to determine an access level for the client device from the set of access levels described by the custom access policy.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A service provider provides flexible access to services using an identity provider. The service provider is associated with a custom access policy used by the identity provider to authenticate access requests associated with client devices for services of the client system. The custom access policy describes a set of access levels corresponding to variable levels of access to services of the service provider. The identity provider authenticates access requests by client devices using one or more device signals from the client devices. In some embodiments, the identity provider determines a device trust score for the client device using the one or more device signals. The identity provider provides an authentication response to the client system based on the custom access policy. The client system uses the authentication response to determine an access level for the client device from the set of access levels described by the custom access policy.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.

Abstract: A multi-tenant system provides software-updating functionality that selectively updates tenant systems according to a number of different factors. In some embodiments, the factors include evaluation of “health metrics” that quantify how well tenant systems are functioning after an update of software components to the latest version, policies specified by either the tenants or the multi-tenant system (or both), and/or “kill switches” specified by the multi-tenant system that prevent updating, as some examples.

Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Abstract: A method including capturing carbon dioxide (CO2) from air (e.g., atmosphere) in an absorber in which the air contacts a base (e.g., a hydroxide, such as potassium hydroxide KOH and/or sodium hydroxide (NaOH)) to produce a carbonate (e.g., potassium carbonate (K2CO3) and/or sodium carbonate (Na2CO3)); precipitating one or more (e.g., carbonate) salt from an aqueous solution comprising salt (a brine) to provide an aqueous solution comprising a chloride (e.g., potassium chloride (KCl) and/or sodium chloride (NaCl)); using electrochemical regeneration to convert the chloride to electrochemically regenerated product comprising the base (e.g., KOH and/or NaOH); and recycling at least a portion of the electrochemically regenerated product comprising the base to the capturing of the CO2 from the air. A system for carrying out the method is also provided.

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. Devices use flow focusing arrangements of channels to produce droplets. The invention further provides multiplex devices that increase droplet formation.

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The invention provides multiplex devices that increase droplet formation in a limited area.

Abstract: A multi-tenant system provides software-updating functionality that selectively updates tenant systems according to a number of different factors. In some embodiments, the factors include evaluation of “health metrics” that quantify how well tenant systems are functioning after an update of software components to the latest version, policies specified by either the tenants or the multi-tenant system (or both), and/or “kill switches” specified by the multi-tenant system that prevent updating, as some examples.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Abstract: A service provider provides flexible access to services using an identity provider. The service provider is associated with a custom access policy used by the identity provider to authenticate access requests associated with client devices for services of the client system. The custom access policy describes a set of access levels corresponding to variable levels of access to services of the service provider. The identity provider authenticates access requests by client devices using one or more device signals from the client devices. In some embodiments, the identity provider determines a device trust score for the client device using the one or more device signals. The identity provider provides an authentication response to the client system based on the custom access policy. The client system uses the authentication response to determine an access level for the client device from the set of access levels described by the custom access policy.

Abstract: A multi-tenant system provides software-updating functionality that selectively updates tenant systems according to a number of different factors. In some embodiments, the factors include evaluation of “health metrics” that quantify how well tenant systems are functioning after an update of software components to the latest version, policies specified by either the tenants or the multi-tenant system (or both), and/or “kill switches” specified by the multi-tenant system that prevent updating, as some examples.