Abstract: A method for preparing a skin regeneration scaffold is disclosed. The method may include preparing a polymer solution by dissolving a biopolymer in a solvent, and subjecting the polymer solution to a template-assisted extrusion process with a nanoporous material as a template in order to produce polymer nanofibers. Furthermore, the method includes fabricating a multilayer composite nanofibrous scaffold using the polymer nanofibers. The composite nanofibrous scaffold may be seeded with cells. In some cases, the cells may be selected from autologous cells, allogeneic cells, or combinations thereof.

Abstract: Methods for preparing and orientating nanofibers and a composite material including the same. Some methods for preparing a composite material with orientated nanofibers may include providing a nanoporous material; dissolving a natural or synthetic polymer, —in a solvent; pressing or drawing the polymer solution through pores of the nanoporous material whereby nanofibers are formed within said material; mixing the nanofibers with a matrix material; orientating or partially orientating the nanofibers within the matrix material by applying an electric and/or magnetic field; depositing the nanofibers-matrix mixture with the orientated or partially orientated nanofibers onto a substrate surface. The nanofibers may be oriented locally different in various areas/layers of the composite material, resulting in a composite material with locally independent mechanical properties.

Abstract: A plurality of initial biological cells is provided, and the initial biological are cultured cells on a pseudo-3D substrate that has a cell-resembling surface to form a plurality of cultured cells. The plurality of cultured cells is subjected to a cell analysis assay. Optionally, the response of the cultured cells to the cell analysis assays is evaluated.