Patents by Inventor Mohammed Zourob

Mohammed Zourob has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11035817
    Abstract: A method and process to make and use cotton-tipped electrochemical immunosensor for the detection of corona viruses is described. The immunosensor were fabricated by immobilizing the virus antigens on carbon nanofiber-modified screen printed electrodes which were functionalized by diazonium electrografting and activated by EDC/NHS chemistry. The detection of virus antigens were achieved via swabbing followed by competitive assay using fixed amount of antibody in the solution. Ferro/ferricyanide redox probe was used for the detection using square wave voltammetric technique. The limits of detection for our electrochemical biosensors were 0.8 and 0.09 pg/ml for SARS-CoV-2 and MERS-CoV, respectively indicating very good sensitivity for the sensors. Both biosensors did not show significant cross reactivity with other virus antigens such as influenza A and HCoV, indicating the high selectivity of the method.
    Type: Grant
    Filed: November 9, 2020
    Date of Patent: June 15, 2021
    Assignee: Alfaisal University
    Inventors: Shimaa Eissa, Mohammed Zourob
  • Patent number: 10962529
    Abstract: A method for detecting a SARS-Cov-2 protease in a biological sample is provided. The method includes contacting the biological sample with a fluorescent probe based sensor, wherein the sensor comprises an L-Histidine-D-aspartic acid peptide substrate, a fluorophore, and a quencher molecule; and detecting the SARS-Cov-2 protease when an increase in fluorescence is observed.
    Type: Grant
    Filed: December 1, 2020
    Date of Patent: March 30, 2021
    Assignee: King Abdulaziz University
    Inventors: Hani A. Alhadrami, Mohammed Zourob
  • Patent number: 10788488
    Abstract: The developed long and short aptamer sequences can be used as high affinity and specificity tools to analyze the drug Dabigatran etexilate for therapeutic drug monitoring, pharmacokinetic and naturalization studies. Using the technique by performing several rounds of selection and enrichment with a randomized 60-mer DNA library, a number of specific aptamer sequences were successfully selected for Dabigatran etexilate. We evaluated the binding affinity and specificity of the generated aptamers showing dissociation constants (kd) ranging from 47-312 nM and very weak or no cross-reactivity to other analytes. Complimentary sequences labelled with a fluorophore and a quencher was used for mapping the binding region within the aptamer by monitoring the change in the fluorescence signal. A truncated sequence was used to construct a turn-on fluorescence sensor.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: September 29, 2020
    Assignee: Alfaisal University
    Inventors: Raja Chinnappan, Mohammed Zourob, Shimaa Eissa, Maher M Aljohani
  • Patent number: 10520462
    Abstract: An electrochemical screening method for the selection of DNA aptamers against 11-deoxycortisol (11-DCL) using gold electrode for target immobilization is described. The gold electrode is used as solid matrix instead of the beads for SELEX. The selection steps (SELEX) are performed on the 11-DC modified electrode directly as the DNA library in the first round or the enriched DNA pools in the subsequent rounds were incubated on the electrode, then the unbound DNA is washed and the bound DNA is measured directly by square wave voltammetry. Then elution of the bound DNA is performed for further use.
    Type: Grant
    Filed: November 24, 2018
    Date of Patent: December 31, 2019
    Assignee: Alfaisal University
    Inventors: Shimaa Eissa, Mohammed Zourob, Raja Chinnappan, Ayesha Siddiqua
  • Patent number: 10436779
    Abstract: There is provided a biosensor for detecting pathogens in a sample. The detection is based on colorimetry. The biosensor comprises one or more particle supports and a magnetic material attached to a planar support. The biosensor embodies magnetic particles that are functionalized using a chemical substrate specific to the pathogens to be detected. The sensor may allow for a simultaneous detection of a plurality of pathogens in the sample. Also, the sensor may be disposable. Moreover, the sensor may be integrated in a portable detection device.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: October 8, 2019
    Assignee: Alfaisal University
    Inventor: Mohammed Zourob
  • Patent number: 10337047
    Abstract: A multiplex hand-held diagnostic biosensor, using two inflammatory salivary biomarkers, Human Neutrophil Elastase (HNE) and Cathepsin-G, was constructed made to potentially detect Periodontitis at an early stage is described. The use of magnetic nanoparticle biosensor method used as a device was based on the measurement of proteolytic activity using specific proteases probes. The magnetic nanoparticle biosensor device is capable of specific and quantitative detection of HNE and Cathepsin-G in solution and in spiked saliva samples with a lower detection limit of 1 pg/mL and 100 fg/mL for HNE and Cathepsin-G, respectively.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: July 2, 2019
    Assignee: Alfaisal University
    Inventor: Mohammed Zourob
  • Patent number: 10151749
    Abstract: There is provided a method of detecting an analyte in a sample. The method is based on colorimetry and also on the binding affinity between the analyte and a chemical substrate which may be a recognition receptor thereof. The method involves a support and a colored carrier. A kit for use in the detection is also provided.
    Type: Grant
    Filed: September 12, 2015
    Date of Patent: December 11, 2018
    Assignee: Alfaisal University
    Inventor: Mohammed Zourob
  • Publication number: 20180031578
    Abstract: High affinity DNA aptamers Seq ID#1-8 for HbA1C and tHb were successfully selected using SELEX after 11 rounds of selection. The tested aptamers bind to HbA1C with dissociation constants in the nanomolar range with the highest affinity aptamer, Seq ID#6, exhibiting a Kd of 2.8 nM. Another aptamer sequence (Seq ID #4) which showed high binding affinity to tHb with a Kd of 2.7 nM was also selected. The HbA1C and tHb-specific aptamers were then applied for the detection of HbA1C % using a voltammetric aptasensor array platform showing remarkable sensitivity and selectivity. The aptasensor array platform was validated using standard human whole blood samples and demonstrated linearity over wide concentration range. The developed platform is superior to current methodologies due to its simplicity, stability and lower cost which will facilitate the early and accurate diagnosis of diabetes.
    Type: Application
    Filed: July 29, 2016
    Publication date: February 1, 2018
    Applicant: Alfaisal University
    Inventors: Shimaa Eissa, MOHAMMED ZOUROB
  • Patent number: 9863962
    Abstract: High affinity DNA aptamers Seq ID#1-8 for HbA1C and tHb were successfully selected using SELEX after 11 rounds of selection. The tested aptamers bind to HbA1C with dissociation constants in the nanomolar range with the highest affinity aptamer, Seq ID#6, exhibiting a Kd of 2.8 nM. Another aptamer sequence (Seq ID #4) which showed high binding affinity to tHb with a Kd of 2.7 nM was also selected. The HbA1C and tHb-specific aptamers were then applied for the detection of HbA1C % using a voltammetric aptasensor array platform showing remarkable sensitivity and selectivity. The aptasensor array platform was validated using standard human whole blood samples and demonstrated linearity over wide concentration range. The developed platform is superior to current methodologies due to its simplicity, stability and lower cost which will facilitate the early and accurate diagnosis of diabetes.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: January 9, 2018
    Assignee: Alfaisal University
    Inventors: Shimaa Eissa, Mohammed Zourob
  • Publication number: 20170038370
    Abstract: There is provided a method of detecting an analyte in a sample. The method is based on colorimetry and also on the binding affinity between the analyte and a chemical substrate which may be a recognition receptor thereof. The method involves a support and a colored carrier. A kit for use in the detection is also provided.
    Type: Application
    Filed: September 12, 2015
    Publication date: February 9, 2017
    Applicant: Alfaisal University
    Inventor: MOHAMMED ZOUROB
  • Publication number: 20170038373
    Abstract: There is provided a biosensor for detecting pathogens in a sample. The detection is based on colorimetry. The biosensor comprises one or more particle supports and a magnetic material attached to a planar support. The biosensor embodies magnetic particles that are functionalized using a chemical substrate specific to the pathogens to be detected. The sensor may allow for a simultaneous detection of a plurality of pathogens in the sample. Also, the sensor may be disposable. Moreover, the sensor may be integrated in a portable detection device.
    Type: Application
    Filed: September 28, 2015
    Publication date: February 9, 2017
    Applicant: Alfaisal University
    Inventor: MOHAMMED ZOUROB
  • Publication number: 20170037450
    Abstract: A multiplex hand-held diagnostic biosensor, using two inflammatory salivary biomarkers, Human Neutrophil Elastase (HNE) and Cathepsin-G, was constructed made to potentially detect Periodontitis at an early stage is described. The use of magnetic nanoparticle biosensor method used as a device was based on the measurement of proteolytic activity using specific proteases probes. The magnetic nanoparticle biosensor device is capable of specific and quantitative detection of HNE and Cathepsin-G in solution and in spiked saliva samples with a lower detection limit of 1 pg/mL and 100 fg/mL for HNE and Cathepsin-G, respectively.
    Type: Application
    Filed: August 5, 2015
    Publication date: February 9, 2017
    Applicant: ALFAISAL UNIVERSITY
    Inventor: MOHAMMED ZOUROB
  • Publication number: 20140220667
    Abstract: There is provided a portable device for detecting pathogens in a sample, the detection being based on an assay involving one or more reagents and heat. The device comprises a flexible substrate having a plurality of spaced apart reservoirs along its length, each reservoir adapted for receiving reagents and the sample, the substrate being secured to a pair of spaced apart reels and movable there between by rolling. Also, the device comprises heating means which can be secured to one of the two reels.
    Type: Application
    Filed: July 22, 2013
    Publication date: August 7, 2014
    Inventors: Minhaz Uddin Ahmed, Mohammed Zourob, Mohammadali Safavieh
  • Publication number: 20090068638
    Abstract: The present invention relates to the field of biosensors useful for detecting bacteria. More particularly, the present invention relates to an electrochemical cell or biosensor and its use in a phage-based method and kit for the detection of bacteria.
    Type: Application
    Filed: July 29, 2008
    Publication date: March 12, 2009
    Applicant: BIOPHAGE INC.
    Inventors: Arghavan SHABANI, Mohammed ZOUROB, Beatrice ALLAIN, Rosemonde MANDEVILLE
  • Patent number: 7492978
    Abstract: A novel single point leaky waveguide structure and its use as an optical sensor for the detection of particles is disclosed. The waveguide structure is fabricated to increase the overlap of the evanescent field extension from the sensor surface with particles in the bulk solution of a flowing system so as to place most of the volume of the particles within the evanescent field. Increasing the overlap of the evanescent field with the particles and permitting mode propagation along the direction of flow for a few millimetres provides an effective interrogation approach for multiple particle detection in a single flow channel.
    Type: Grant
    Filed: October 7, 2002
    Date of Patent: February 17, 2009
    Assignee: The Secretary of State for Defence
    Inventors: Mohammed Zourob, Stephan Mohr, Bernard James Treves Brown, Peter Robert Fielden, Nicholas John Goddard
  • Publication number: 20060228257
    Abstract: Apparatus for detecting particles in a liquid, comprises an evanescent optical sensor having an electrically conducting layer, and means associated therewith for providing an electric field at or adjacent the sensor surface. Preferably, the optical sensor is a surface bound sensor based on a metal-clad leaky waveguide structure. A DC potential applied to the metal layer and a parallel counter electrode provides a uniform electric field, which drives deposition of the particles on the sensor surface. Alternatively, an alternating potential applied to a electrically conducting layer configured as an electrode and counter electrode induces an electro-osmotic bulk flow in the liquid which drives deposition of the particles on the sensor surface.
    Type: Application
    Filed: February 12, 2004
    Publication date: October 12, 2006
    Inventors: Michael Hughes, Kai Hoettges, Martin McDonnell, Nicholas Goddard, Mohammed Zourob
  • Publication number: 20060147147
    Abstract: A novel single point leaky waveguide structure and its use as an optical sensor for the detection of particles is disclosed. The waveguide structure is fabricated to increase the overlap of the evanescent field extension from the sensor surface with particles in the bulk solution of a flowing system so as to place most of the volume of the particles within the evanescent field. Increasing the overlap of the evanescent field with the particles and permitting mode propagation along the direction of flow for a few millimetres provides an effective interrogation approach for multiple particle detection in a single flow channel.
    Type: Application
    Filed: October 7, 2002
    Publication date: July 6, 2006
    Inventors: Mohammed Zourob, Stephan Mohr, Bernard Treves Brown, Peter Fielden, Micholas Goddard