Abstract: The present invention pertains to a method to isolate, separate, enrich or amplify a targeted nucleotide polymer such as mRNA through selective reverse transcription of the targeted polymer into cDNA from a sample comprising of chemically identical or similar polynucleotide polymers such as rRNA. The enrichment of the targeted nucleic acid such as mRNA is accomplished by blocking the reverse transcription of undesired rRNA while allowing unrestricted reverse transcription of the targeted polymer. The invention also embodies that the cleavage of the non-targeted nucleic acid such as rRNA bound to an oligonucleotide through enzymatic activity (RNase H).

Abstract: A nucleic acid based method is provided for the detection of methanogens in human, animal, plant and in environmental samples of soil, sediment or water that are terrestrial or subterranean in origin. The method is effected by (a) obtaining a biological sample; and (b) analyzing the sample for a nucleic acid sequence/s unique to methanogens, wherein a detectable level of the nucleic acid sequence unique to methanogens is indicative of the presence of methanogens in the sample. Further, a scheme for inferring the identity of the different types of methanogens is provided, wherein, the DNA sequences of the methyl reductase genes detected in that sample are compared to methyl reductase sequences of known methanogens. With this technology, methanogens in samples containing less than {fraction (1/1000)}th of a gram of biomass can be detected.

Abstract: A process is disclosed for stimulating the activity of microbial consortia in a subterranean formation to convert hydrocarbons to methane, which can be produced. Fluid and rock of the formation are analyzed. The presence of microbial consortia is determined and a characterization made (preferably genetic) of at least one microorganism of the consortia, at least one being a methanogenic microorganism. The characterization is compared with at least one known characterization derived from a known microorganism having one or more known physiological and ecological characteristics. This information, together with the information obtained from the analysis of the fluid and rock, is used to determine an ecological environment that promotes in situ microbial degradation of formation hydrocarbons and promotes microbial generation of methane by at least one methanogenic microorganism of the consortia. This information is then used as the basis for modifying the formation environment to produce methane.

Abstract: A process is disclosed for stimulating the activity of microbial consortia in a subterranean formation to convert hydrocarbons to methane, which can be produced. Fluid and rock of the formation are analyzed. The presence of microbial consortia is determined and a characterization made (preferably genetic) of at least one microorganism of the consortia, at least one being a methanogenic microorganism. The characterization is compared with at least one known characterization derived from a known microorganism having one or more known physiological and ecological characteristics. This information, together with the information obtained from the analysis of the fluid and rock, is used to determine an ecological environment that promotes in situ microbial degradation of formation hydrocarbons and promotes microbial generation of methane by at least one methanogenic microorganism of the consortia. This information is then used as the basis for modifying the formation environment to produce methane.