Abstract: A method comprising obtaining a substantially cell-free sample of blood plasma or blood serum from a subject with osteoarthritis; and detecting a presence of or measuring a level of novel_miRNA_1 (gucuggcucaggguuggg) (SEQ ID NO: 1), novel_miRNA_2 (ucccuguucgggcgccacu) (SEQ ID NO: 2), novel_miRNA_3 (uguuuagcauccuguagccugc) (SEQ ID NO: 3), and novel_miRNA_4 (uaguggguuaucagaacu) (SEQ ID NO: 4). Also provided are methods where additional miRNAs are detected including novel miRNA 5 (SEQ ID NO: 5), novel miRNA 6 (SEQ ID NO: 6), novel miRNA 7 (SEQ ID NO: 7), novel miRNA 8 (SEQ ID NO: 8), novel miRNA 9 (SEQ ID NO: 9), novel miRNA 10 (SEQ ID NO: 10), novel miRNA 11 (SEQ ID NO: 11), novel miRNA 12 (SEQ ID NO: 12), novel miRNA 13 (SEQ ID NO: 13), hsa-miR-335-3p, hsa-miR-199a-5p, hsa-miR-671-3p, hsa-miR-1260b, hsa-miR-191-3p, hsa-miR-335-5p and/or hsa-miR-543.

Abstract: A method staging of knee osteoarthritis (OA) comprising a) obtaining a substantially cell-free sample of blood plasma or blood serum from a subject with osteoarthritis; b) detecting a presence of or measuring a level of one or more miRNAs selected from hsa-miR-335-3p, hsa-miR-199a-5p, hsa-miR-671-3p, hsa-miR-1260b, hsa-miR-191-3p, hsa-miR-335-5p, hsa-miR-543, novel_miRNA_1 (gucuggcucaggguuggg) (SEQ ID NO: 1), novel_miRNA_2 (ucccuguucgggcgccacu) (SEQ ID NO: 2), novel_miRNA_3 (uguuuagcauccuguagccugc) (SEQ ID NO: 3), and novel_miRNA_4 (uaguggguuaucagaacu) (SEQ ID NO: 4); and c) identifying the subject as likely to have early stage osteoarthritis or late stage osteoarthritis based on the presence of or measured level of the one or more miRNAs. Isolated nucleic acids, primers, probes, panels and kits are also provided.

Abstract: There is disclosed herein methods, uses and systems for the detection, diagnosis, prognosis, treatment or prevention of a disease or condition comprising cartilage degeneration in a subject that is in need thereof. The methods comprise the use, inhibition or measurement of at least one of miR-181 a-5p and miR-4454, in the subject.

Abstract: Methods of synthesizing compounds using CO2 as a directing group for C—H functionalization, and compounds made thereby, are described.

Abstract: Methods of synthesizing compounds using CO2 as a directing group for C—H functionalization, and compounds made thereby, are described.

Abstract: There is disclosed herein methods, uses and systems for the detection, diagnosis, prognosis, treatment or prevention of a disease or condition comprising cartilage degeneration in a subject that is in need thereof. The methods comprise the use, inhibition or measurement of at least one of miR-181 a-5p and miR-4454, in the subject.

Abstract: An end-to-end, centralized and globally-accessible platform for work assignment input, allocation, performance and tracking is herein described. The platform is configured to improve operational and quality assurance effectiveness and efficiency through automated work allocation, performance tracking and reporting. The end-to-end approach accounts for work assignment input, work assignment allocation, work assignment processing and quality assessment and assurance of the completed work assignments. In addition, both automated and manual work assignment allocations from both an operation (i.e., work input and allocation) perspective, as well as, a quality assurance perspective are possible.

Abstract: Systems, apparatus, and computer program products provide for a comprehensive quality assurance platform for automatically assessing the quality of work performance of associates. The described quality assurance system/module that can be implemented in conjunction with the work allocation system described herein or the system can operate as a standalone quality assurance system/module. The quality assurance system provides for automated collection and tracking of quality metrics and automated quality calibrations (i.e., automated and random selection of work assignment/transactions for comparison to standards). Additionally, the quality assurance system provides for validating the quality assurance work by random sampling and quality assurance assessment of the auditor's work and an associate improvement plan, whereby quality assurance metrics may be systematically adjusted and/or sampling sizes increased for associates with quality scores that dictate a need for improvement.