Abstract: Support for a product may be provided using an interactive chat interface. The interface may provide recommendations for actions the user may take to address the issue. A support assistant tree may be used in providing information to the user via the user interface. The user interface may include options selectable by the user to traverse the support assistant tree. For example, a server may initially record the state of the user as being at the root node of the support assistant tree. Each child node of the root node may be associated with an answer to a question or an option. When the user selects the associated answer or option, the server provides information related to the corresponding node and updates the recorded state of the user to be the corresponding node. The process may be repeated until a leaf node is reached.

Abstract: Compositions, kits, and systems are disclosed that include calreticulin gene delivering nanoparticles that can be used alone or in combination with focused ultrasound to induce immunogenic cell death in tumors. Also disclosed are methods of producing and using the compositions, kits, and systems.