Abstract: The present invention relates to a method of preventing and/or treating diabetes type 2, also, it relates to a method of augmenting Glut4 phosphorylation and Glut4 translocation to a target cell membrane to enhance insulin signal in a signal transduction pathway; further, it relates to a simplified and inexpensive process of obtaining extract and thereafter selectively, its active n-butanol fraction and active molecule Lupinoside PA (LPA4), useful in preventing and/or treating diabetes type 2; and lastly, a pharmaceutical composition thereof.

Abstract: The present invention relates to an adipocyte Insulin adpInsl with Insulin A and B chains of SEQ ID Nos. 1 and 2, a effective method of treating type 2 diabetes in a subject using adipocyte insulin, said method comprising steps of administering the insulin to a diabetic intraperitoneally, an Insulin gene of SEQ ID No. 3, a process of isolating protein Insulin from the adipocytes of the Carp, said method comprising steps of reverse transcripting RNA of adipocytes to obtain cDNA, using oligonucleotide primers of SEQ ID Nos. 5 and 6 to identify AdpInsl gene from cDNA, and deducing amino acid sequence from cDNA to obtain protein Insulin, and an adipocyte of Catla Catla useful for producing the said Insulin.

Abstract: The present invention relates to a method of preventing and/or treating diabetes type 2, also, it relates to a method of augmenting Glut4 phosphorylation and Glut4 translocation to a target cell membrane to enhance insulin signal in a signal transduction pathway; further, it relates to a simplified and inexpensive process of obtaining extract and thereafter selectively, its active n-butanol fraction and active molecule Lupinoside PA (LPA4), useful in preventing and/or treating diabetes type 2; and lastly, a pharmaceutical composition thereof.

Abstract: The present invention relates to a novel sperm immotility protein isolated from an earthworm, Pheretima posthuma, belonging to the phylum, Annelida useful as an anti fertility agent, and a method for the isolation of an active factor in the Coelomic Fluid (CF) of commonly available mature specimens of the earthworm, Pheretima posthuma belonging to the phylum, Annelida useful as an anti fertility agent.

Abstract: The present invention relates to an adipocyte Insulin adplnsl with Insulin A and B chains of SEQ ID Nos. 1 and 2, a effective method of treating type 2 diabetes in a subject using adipocyte insulin, said method comprising steps of administering the insulin to a diabetic intraperitoneally, an Insulin gene of SEQ ID No. 3, a process of isolating protein Insulin from the adipocytes of the Carp, said method comprising steps of reverse transcripting RNA of adipocytes to obtain cDNA, using oligonucleotide primers of SEQ ID Nos. 5 and 5 to identify AdpInsl gene from cDNA, and deducing amino acid sequence from cDNA to obtain protein Insulin, and an adipocyte of Catla Catla useful for producing the said Insulin.

Abstract: A sperm immotility and anti-fertility agent is provided. The agent comprises coelomic fluid extract which is obtained from an Indian earthworm, Pheretima posthuma, belonging to the phylum, Annelida. The agent may further comprise pharmaceutically acceptable additives and can be in the form of a solution, gel, powder, capsules, tablets or cream. The agent is a non-steroid and also can be used externally. Upon further purification a protein can be isolated having a molecular weight of 20 kilodaltons. This protein is anionic and non-toxic and also is without hemolytic activity. Also the protein as well as the extract can provide 100% sperm immotility within 2 minutes. In addition, methods of isolation of an active factor of the coelomic fluid, determination of toxicity and hemolytic activity of the agent as well as preparing a sperm suspension from male rats and mice useful for bio-assay are disclosed.