Abstract: Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.

Abstract: A mobile smart container system comprises a housing, an access component configured to secure access to a compartment within the housing when in a closed position, a communication interface configured to wirelessly receive a request to access the compartment, a perceivable output device, an electromechanical latch configured to engage with the access component to releasably lock the access component in the closed position, and a processor. The processor receives and authenticates the request to access the compartment and, in response to receiving and authenticating the request, activates the electromechanical latch to unlock the access component to make the compartment accessible, and outputs, upon actuation of the electromechanical latch, an alert via the perceivable output device to identify the smart container system.

Abstract: A bed covering system includes a top cover having a top surface, a bottom surface, right, left, top and bottom edges, and at least one fold on at least part of the bottom surface; a bottom cover having a top surface, a bottom surface, right, left, top and bottom edges, and at least one fold on at least part of the top surface; and a concealed fastening means having a first fastener part and a second fastener part, wherein the first and second fastener parts are attached to and enclosed in the at least one fold of the top and bottom covers, respectively. The top and bottom covers are detachably connected by the first and second fastener parts. A bed covering system also includes a plurality of covers and a plurality of fastening means. Teaching methods using the bed covering systems are included.