Abstract: The present invention provides a method to advertise consumer items that are used in outdoor activities. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of a product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. The system can be used to advertise recreational events and commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sunglasses, surfboards, bicycles, wetsuits, cell phones, computers, golf clubs, golf balls, fishing poles, and lures.

Abstract: The present invention provides a method to personalize and enhance photo documents. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of a photo document to a unique event by the consumer will lead to an enhanced value of the product. Photo documents containing RFID tags are associated with personal images, movies, text, and audio files. Information that is stored on cell phones, PDAs, computers, and external databases is transferred and shared using cellular networks, email, text, and multimedia messaging. This invention describes a system to record, retrieve, and share personal experiences and event information in association with commercial products such as real estate properties.

Abstract: The present invention provides a method to advertise real estate, events, and consumer items. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of a product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. The system can be used to advertise real estate, recreational events, and commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sun glasses, surfboards, bicycles, wetsuits, cell phones, computers, trading cards, golf clubs, golf balls, fishing poles, and lures.

Abstract: The present invention provides a method to personalize and enhance the value of consumer items for use in outdoor activities. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of the product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. Commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sun glasses, surfboards, bicycles, wetsuits, cell phones, computers, golf clubs, golf balls, fishing poles, and lures will be produced that contain RFID tags.

Abstract: The present invention provides a method to personalize and enhance the value of consumer items for use in outdoor activities. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of the product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. Commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sun glasses, surfboards, bicycles, wetsuits, cell phones, computers, golf clubs, golf balls, fishing poles, and lures will be produced that contain RFID tags.

Abstract: This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2. The invention is further directed to a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant host, and a method of determining the presence of a trait in a plant host, using a monopartite or bipartite viral vector derived from a tobravirus.

Abstract: The present invention provides a method to advertise consumer items that are used in outdoor activities. It utilizes radio frequency identification (RFID) tags, computers, PDAs, and cell phones to link, record, and retrieve positional, personal, and historical data. The personalization and association of an object to a unique event by the consumer will lead to enhanced value of a product. Information that is stored on cell phones, PDAs, computers, and external databases are transferred as multimedia messages. The information is cataloged and organized using the unique RFID number. The subject line of the multimedia message is the RFID tag number. The system can be used to advertise recreational events and commercial items such as skis, coats, hats, shoes, shirts, pants, gloves, sunglasses, surfboards, bicycles, wetsuits, cell phones, computers, golf clubs, golf balls, fishing poles, and lures.

Abstract: The present invention relates to a method for conferring herbicide, pest, and disease resistance in plant hosts. Specifically, the present invention employs transient viral expression vectors to express proteins or enzymes conferring resistance in plant hosts. In addition, a library of nucleotide sequence variants in a sense or antisene orientation may be used to determine the targets of an herbicide or pathogen and to screen suitable viral nucleic acids for herbicide, pest, and disease resistance.

Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.

Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.

Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.

Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human a galactosidase nucleotide sequences.

Abstract: The present invention provides a method for enhancing the production of RNAs or proteins in a plant host using either non-native 5′ untranslated sequences or artificial leader sequences. Preferably, commercially useful proteins, polypeptides, or fusion products thereof are produced, such as, enzymes, antibodies, hormones, pharmaceuticals, vaccines, pigments, anti-microbial polypeptides, and the like. The non-native 5′ untranslated enhancers may also be effective in many different types of transcription or translation systems, such as bacterial and animal systems.

Abstract: The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region.

Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.

Abstract: This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2. The invention is further directed to a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant host, and a method of determining the presence of a trait in a plant host, using a monopartite or bipartite viral vector derived from a tobravirus.

Abstract: The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region.

Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.

Abstract: This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2.

Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human &agr; galactosidase nucleotide sequences.