Abstract: The present invention relates to a Lactiplantibacillus plantarum KM2 strain isolated from aged meat, and a use thereof, wherein the strain has proteolytic activity, immunity-enhancing activity, and probiotic properties, wherein it was found that the Lactiplantibacillus plantarum KM2 strain exhibits proteolytic activity and an immunity-enhancing effect, and does not exhibit hemolytic activity or have genes related to harmful substances or harmful enzymes. Thus, the Lactiplantibacillus plantarum KM2 strain can be used as a material for various probiotics, functional food compositions, and pharmaceutical compositions.

Abstract: The present invention relates to a composition containing bioconverted black soybean powder of which various biologically active functions are improved by treating whole soy milk with an enzyme solution derived from Bacillus polyfermenticus strain. The present invention has identified that low-molecular-weight functional amino acids and peptides are formed by treating whole soy milk with a fermentation solution in which various peptide synthetases and lyases derived from a Bacillus polyfermenticus strain are mixed, and that bioconverted black soybean powder has functional effects such as antioxidant activity and behavioral disorder improvement, and thus a composition for alleviating behavioral disorders, containing bioconverted black soybean powder as an active ingredient, is provided.

Abstract: The present invention relates to a method for preparing bioconversion powder having various improved physiological activity functions, by treatment with an enzyme solution derived from a Bacillus polyfermenticus strain, and provides a method for preparing bioconversion powder using a fermentation broth of a Bacillus polyfermenticus strain, by confirming that low-molecular-weight amino acids, peptides and various functional ingredients were formed by treating whole soy milk with a fermentation broth mixed with various degrading enzymes and peptide-synthesizing enzymes derived from the Bacillus polyfermenticus strain.

Abstract: The present invention relates to a novel halophilic Bacillus amyloliquefaciens KMUS1 strain (KCTC13635BP) producing thrombolytic enzyme nattokinase, and the halophilic Bacillus amyloliquefaciens KMUS1 strain according to the present invention produces highly active nattokinase. When food is fermented using the above strain, it can also be used in foods with high salt concentration. In addition, it can be used in the food industry through the role of dissolving and preventing blood clots by nattokinase produced by the strain.

Abstract: The present invention relates to a halophilic Bacillus polyfermenticus KMU01 strain producing salt-tolerant gamma-glutamyl transpeptidase, and the halophilic Bacillus polyfermenticus KMU01 strain according to the present invention (KCTC11751BP) producing a halophilic gamma-glutamyl transpeptidase, wherein, when food is fermented using the strain of the present invention, it can be utilized in foods with high salt concentration, and fermented foods having excellent flavor and various peptides can be prepared by high enzyme activity that decomposes protein binding.

Abstract: The present invention relates to a method of preparing a poly-?-glutamic acid hydrogel using ultraviolet ray and to a use of the poly-?-glutamic acid hydrogel prepared by the method, and the method of preparing poly-?-glutamic acid hydrogel using ultraviolet irradiation according to the present invention solved the problem of microbial contamination in the poly-?-glutamic acid solution, and produced poly-?-glutamic acid hydrogel in high yield by only a simple treatment process, and as it was confirmed that the poly-?-glutamic acid hydrogel has improved storage stability in a solution, the poly-gamma-glutamic acid hydrogel prepared by the method of preparing the same of the present invention can be provided as a tissue engineering scaffold, artificial organs and bio-ink for 3D printing.

Abstract: The present invention relates to a method of preparing a poly-?-glutamic acid hydrogel using ultraviolet ray and to a use of the poly-?-glutamic acid hydrogel prepared by the method, and the method of preparing poly-?-glutamic acid hydrogel using ultraviolet irradiation according to the present invention solved the problem of microbial contamination in the poly-?-glutamic acid solution, and produced poly-?-glutamic acid hydrogel in high yield by only a simple treatment process, and as it was confirmed that the poly-?-glutamic acid hydrogel has improved storage stability in a solution, the poly-gamma-glutamic acid hydrogel prepared by the method of preparing the same of the present invention can be provided as a tissue engineering scaffold, artificial organs and bio-ink for 3D printing.

Abstract: The present invention relates to a composition for shielding radiation comprising biomaterial poly-gamma-glutamic acid, and it is confirmed that the polygamma-glutamic acid polymer exhibits a radiation shielding effect up to 87% at a concentration of 0.33 to 1.65 ?M and thus a composition comprising the poly-gamma-glutamic acid is provided as a material for shielding radiation, and a radiation shielding material using the composition and a method of preparing the same are provided.

Abstract: A method of stabilizing a membrane protein includes forming a membrane protein-amphiphilic polymer complex by binding a membrane protein in an aqueous solution to the amphiphilic polymer represented by Formula 1. The amphiphilic polymer includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution.

Abstract: A method of stabilizing a membrane protein includes forming a membrane protein-amphiphilic polymer complex by binding a membrane protein in an aqueous solution to the amphiphilic polymer represented by Formula 1. The amphiphilic polymer includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution.

Abstract: The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

Abstract: The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

Abstract: The present invention relates to nanomicelles composed of a complex of a lipophilic compound and a poly-gamma-glutamic acid wherein a portion of carboxyl groups are substituted with an amine group, and more particularly, to nanomicelles composed of a complex of a lipophilic compound and a poly-gamma-glutamic acid wherein a portion of carboxyl groups are substituted with an amine group, a preparation method thereof, and a drug delivery system employing the mucous membrane-adhesive property of the nanomicelles. According to the present invention, the nanomicelle drug delivery system based on poly-gamma-glutamic acid that is a natural biopolymer can be used for the delivery of a drug to mucous membranes to thereby increase the in vivo stability and effectiveness of the drug.

Abstract: The present invention relates to an adjuvant composition containing poly-gamma-glutamic acid-chitosan nanoparticles and a vaccine composition containing the adjuvant composition, and more particularly to an adjuvant composition containing nanoparticles prepared by ionic bonding between poly-gamma-glutamic acid having ensured safety and chitosan, and a vaccine composition containing the poly-gamma-glutamic acid-chitosan nanoparticles and an antigen. The adjuvant containing the poly-gamma-glutamic acid-chitosan nanoparticles has little or no toxicity and side effects and is added to human or animal vaccines for the prevention and treatment of viral and bacterial infections and cancers to increase the production of antibodies.

Abstract: The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

Abstract: A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.

Abstract: The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (?-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a ?-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (?-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof. The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (?PGA) and cationic chitosan.

Abstract: The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (?-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient. The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

Abstract: The present invention relates to a hyaluronidase inhibitor containing poly-gamma-glutamic acid (PGA) as an active ingredient, a composition for maintaining skin elasticity and a composition for improving allergy, wherein each of the compositions contains PGA as an active ingredient. The inventive compositions containing PGA are effective in maintaining skin moisturization and skin elasticity by effectively inhibiting the activity of hyaluronidase which is an enzyme that degrades hyaluronic acid present in the skin dermis. Also, the compositions can relieve allergic symptoms by inhibiting the permeability of inflammatory cells.

Abstract: The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (?-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a ?-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (?-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof. The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (?PGA) and cationic chitosan.