Abstract: A probe, a method of manufacturing the probe and a probe card having the probe are disclosed. The probe includes a first connecting member, a body and a tip. Specially, the body integrally includes a bump and a beam. The body is fixed to an electric component by the first connecting member. In addition, the probe card may include a printed circuit board and the electric component as well as the above probe. Here, the probe is independently formed. The probe is then fixed to the electric component so that damage to the electric component may be effectively prevented.

Abstract: The present invention provides an apparatus for amplifying a polynucleotide, comprising a substrate, a microflow channel system disposed in the substrate and comprising a sample inlet port, a sample flow channel extending from the sample inlet port, and a polynucleotide polymerization reaction chamber in fluid communication with the sample flow channel, a first insulation groove formed around the reaction chamber, and a means for regulating a temperature of the reaction chamber. Accordingly, a multiple chamber device for amplifying a polynucleotide, comprising multiple polymerization reaction chambers formed in a substrate can be manufactured.