Abstract: The present disclosure relates to a microbiome composition for improving muscle strength using a heat-treated fermented culture complex of KM2 Lactiplantibacillus plantarum, wherein the composition according to the present disclosure increased synthesis of myoproteins and showed an effect of inhibiting degradation of myoproteins and animal tests showed an effect of improving muscle strength and increasing leg muscle mass through the increase in grip strength, travel distance, and speed, as well as results in which hamstring muscle strength is improved and muscle mass increases even in a clinical trial. In addition, it was found that the beneficial bacteria increased through an increase in the diversity of intestinal microflora that changed with muscle reduction and improvement in the microflora composition, while the harmful bacteria decreased.

Abstract: The present disclosure relates to a microbiome composition of a halophilic Bacillus polyfermenticus KMU01 strain fermented culture supernatant with an anti-obesity effect. It was found that a fermented culture supernatant of the strain deposited in deposit number KCTC11751BP suppresses generation and accumulation of fat and reduces a content of blood cholesterol, such that the fermented culture supernatant may be useful as a composition for preventing, treating, or ameliorating obesity; or a composition for reducing body fat (visceral fat) or blood cholesterol.

Abstract: The present invention relates to a composition containing bioconverted black soybean powder of which various biologically active functions are improved by treating whole soy milk with an enzyme solution derived from Bacillus polyfermenticus strain. The present invention has identified that low-molecular-weight functional amino acids and peptides are formed by treating whole soy milk with a fermentation solution in which various peptide synthetases and lyases derived from a Bacillus polyfermenticus strain are mixed, and that bioconverted black soybean powder has functional effects such as antioxidant activity and behavioral disorder improvement, and thus a composition for alleviating behavioral disorders, containing bioconverted black soybean powder as an active ingredient, is provided.

Abstract: The present invention relates to a Lactiplantibacillus plantarum KM2 strain isolated from aged meat, and a use thereof, wherein the strain has proteolytic activity, immunity-enhancing activity, and probiotic properties, wherein it was found that the Lactiplantibacillus plantarum KM2 strain exhibits proteolytic activity and an immunity-enhancing effect, and does not exhibit hemolytic activity or have genes related to harmful substances or harmful enzymes. Thus, the Lactiplantibacillus plantarum KM2 strain can be used as a material for various probiotics, functional food compositions, and pharmaceutical compositions.

Abstract: The present invention relates to a method for preparing bioconversion powder having various improved physiological activity functions, by treatment with an enzyme solution derived from a Bacillus polyfermenticus strain, and provides a method for preparing bioconversion powder using a fermentation broth of a Bacillus polyfermenticus strain, by confirming that low-molecular-weight amino acids, peptides and various functional ingredients were formed by treating whole soy milk with a fermentation broth mixed with various degrading enzymes and peptide-synthesizing enzymes derived from the Bacillus polyfermenticus strain.

Abstract: The present invention relates to a composition for shielding radiation comprising biomaterial poly-gamma-glutamic acid, and it is confirmed that the polygamma-glutamic acid polymer exhibits a radiation shielding effect up to 87% at a concentration of 0.33 to 1.65 ?M and thus a composition comprising the poly-gamma-glutamic acid is provided as a material for shielding radiation, and a radiation shielding material using the composition and a method of preparing the same are provided.

Abstract: A method of stabilizing a membrane protein includes forming a membrane protein-amphiphilic polymer complex by binding a membrane protein in an aqueous solution to the amphiphilic polymer represented by Formula 1. The amphiphilic polymer includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution.

Abstract: A method of stabilizing a membrane protein includes forming a membrane protein-amphiphilic polymer complex by binding a membrane protein in an aqueous solution to the amphiphilic polymer represented by Formula 1. The amphiphilic polymer includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution.

Abstract: The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

Abstract: The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

Abstract: The present invention relates to nanomicelles composed of a complex of a lipophilic compound and a poly-gamma-glutamic acid wherein a portion of carboxyl groups are substituted with an amine group, and more particularly, to nanomicelles composed of a complex of a lipophilic compound and a poly-gamma-glutamic acid wherein a portion of carboxyl groups are substituted with an amine group, a preparation method thereof, and a drug delivery system employing the mucous membrane-adhesive property of the nanomicelles. According to the present invention, the nanomicelle drug delivery system based on poly-gamma-glutamic acid that is a natural biopolymer can be used for the delivery of a drug to mucous membranes to thereby increase the in vivo stability and effectiveness of the drug.

Abstract: The present invention relates to an adjuvant composition containing poly-gamma-glutamic acid-chitosan nanoparticles and a vaccine composition containing the adjuvant composition, and more particularly to an adjuvant composition containing nanoparticles prepared by ionic bonding between poly-gamma-glutamic acid having ensured safety and chitosan, and a vaccine composition containing the poly-gamma-glutamic acid-chitosan nanoparticles and an antigen. The adjuvant containing the poly-gamma-glutamic acid-chitosan nanoparticles has little or no toxicity and side effects and is added to human or animal vaccines for the prevention and treatment of viral and bacterial infections and cancers to increase the production of antibodies.

Abstract: The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

Abstract: A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.

Abstract: The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (?-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a ?-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (?-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof. The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (?PGA) and cationic chitosan.

Abstract: The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (?-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient. The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

Abstract: The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (?-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a ?-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (?-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof. The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (?PGA) and cationic chitosan.

Abstract: The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

Abstract: A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus. An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

Abstract: The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.