Patents by Inventor Morassa Mohseni Middleton

Morassa Mohseni Middleton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • SERIAL FORMATION OF TERNARY COMPLEX SPECIES
    Publication number: 20220372552
    Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.
    Type: Application
    Filed: June 21, 2022
    Publication date: November 24, 2022
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Brittany A. ROHRMAN, Denis MALYSHEV, Morassa Mohseni MIDDLETON, Arnold OLIPHANT
  • METHODS AND COMPOSITIONS FOR STABILIZING NUCLEIC ACID-NUCLEOTIDE-POLYMERASE COMPLEXES
    Publication number: 20220290216
    Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
    Type: Application
    Filed: January 4, 2022
    Publication date: September 15, 2022
    Applicant: Omniome, Inc.
    Inventors: Morassa Mohseni MIDDLETON, Mark C. WALLEN, Pinar IYIDOGAN, Michael James SCHMIDT, Brittany A. ROHRMAN, Ying Lin LIU, Fabian BLOCK, Arnold OLIPHANT
  • Serial formation of ternary complex species
    Patent number: 11421262
    Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: August 23, 2022
    Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
    Inventors: Brittany A. Rohrman, Denis Malyshev, Morassa Mohseni Middleton, Arnold Oliphant
  • Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
    Patent number: 11242557
    Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: February 8, 2022
    Assignee: OMNIOME, INC.
    Inventors: Morassa Mohseni Middleton, Mark C. Wallen, Pinar Iyidogan, Michael James Schmidt, Brittany A. Rohrman, Ying Lin Liu, Fabian Block, Arnold Oliphant
  • SERIAL FORMATION OF TERNARY COMPLEX SPECIES
    Publication number: 20200032317
    Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.
    Type: Application
    Filed: July 22, 2019
    Publication date: January 30, 2020
    Applicant: Omniome, Inc.
    Inventors: Brittany A. ROHRMAN, Denis MALYSHEV, Morassa Mohseni MIDDLETON, Arnold OLIPHANT
  • METHODS AND COMPOSITIONS FOR STABILIZING NUCLEIC ACID-NUCLEOTIDE-POLYMERASE COMPLEXES
    Publication number: 20190345544
    Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
    Type: Application
    Filed: July 19, 2019
    Publication date: November 14, 2019
    Applicant: Omniome, Inc.
    Inventors: Morassa Mohseni MIDDLETON, Mark C. WALLEN, Pinar IYIDOGAN, Michael James SCHMIDT, Brittany A. ROHRMAN, Ying Lin LIU, Fabian BLOCK, Arnold OLIPHANT
  • Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
    Patent number: 10400272
    Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
    Type: Grant
    Filed: March 15, 2019
    Date of Patent: September 3, 2019
    Assignee: OMNIOME, INC.
    Inventors: Morassa Mohseni Middleton, Mark C. Wallen, Pinar Iyidogan, Michael James Schmidt, Brittany A. Rohrman, Ying Lin Liu, Fabian Block, Arnold Oliphant