Abstract: The invention relates to a novel functional transposase system. More specifically, the invention relates to a DNA transposon comprising a heterologous polynucleotide flanked by transposon flanking regions and to a recombinant transposase of Acyrthosiphon pisum (AP) or Aphis craccivora (AC) comprising an amino acid sequence of at least 90% sequence identity with SEQ ID NO: 4 fused to a heterologous nuclear localization signal, optionally comprising at least one activity improving mutation and to a polynucleotide or an expression vector encoding said DNA transposon or transposase as well as an expression system comprising the transposase/transposon pair. The invention also relates to methods for stably integrating a polynucleotide into cells or stably expressing a protein of interest using the transposase/transposon pair.

Abstract: Aspects of this invention, inter alia, relate to novel systems for targeting, editing or manipulating DNA in a cell, using novel synthetic RNA-guided nucleases (sRGNs). The sRGNs are derived from wildtype or parental small type II CRISPR Cas9 endonucleases.

Abstract: The invention relates to a modified mammalian glutamine synthetase comprising a mutation at amino acid position 10 and/or 298 in a mammalian glutamine synthetase, wherein the mutation is selected from the group consisting of R298K, N10S, N10T and N10Q and to an expression vector, a nucleic acid and a eukaryotic host cell encoding said modified mammalian glutamine synthetase and to its use as a selection marker. The invention further relates to methods for preparing stable cell lines, or for producing a protein of interest using said modified mammalian glutamine synthetase for selection.

Abstract: Aspects of this invention, inter alia, relate to novel systems for targeting, editing or manipulating DNA in a cell, using novel synthetic RNA-guided nucleases (sRGNs). The sRGNs are derived from wildtype or parental small type II CRISPR Cas9 endonucleases.

Abstract: A method for checking an opening and/or closing movement of a motor vehicle body system, including energizing an electric drive device of the vehicle body system such that a movement of the vehicle body system is driven from a closed to open position or open to closed position, and measuring a drive current signal during energizing. The method further includes checking whether predefined or predefinable current changes given by current gradients and amplitudes occur in the drive current signal and whether the predefined or predefinable current changes exceed or undershoot a predefined or predefinable value during the opening and/or closing operation of the vehicle body system, and/or whether a predefined or predefinable current limit value is exceeded or undershot in the drive current signal during a pinching event within a predefined or predefinable path and/or time interval during the opening and/or closing operation of the vehicle body system.

Abstract: Aspects of this invention inter alia relate to novel systems for targeting, editing or manipulating DNA in a cell, comprising one or more heterologous vector(s) encoding a SluCas9 nuclease from Staphylococcus lugdunensis or variants thereof, and one or more guide RNAs (gRNAs), or a SluCas9 nuclease or variant thereof and one or more gRNAs.

Abstract: Described herein are novel systems for targeting, editing or manipulating DNA in a cell, using novel M-SmallCas9 nucleases and variants thereof. The M-SmallCas9 nucleases are derived from wildtype or parental small type II CRISPR Cas9 endonucleases, and display improved fidelity compared to parental type II CRISPR Cas9 enzymes in combination with a simple PAM sequences and are small endonuclease size.

Abstract: Aspects of this invention, inter alia, relate to novel systems for targeting, editing or manipulating DNA in a cell, using novel synthetic RNA-guided nucleases (sRGNs). The sRGNs are derived from wildtype or parental small type II CRISPR Cas9 endonucleases.

Abstract: Aspects of this invention inter alia relate to novel systems for targeting, editing or manipulating DNA in a cell, comprising one or more heterologous vector(s) encoding a SluCas9 nuclease from Staphylococcus lugdunensis or variants thereof, and one or more guide RNAs (gRNAs), or a SluCas9 nuclease or variant thereof and one or more gRNAs.