Patents by Inventor Morten Elde
Morten Elde has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200332352Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: ApplicationFiled: August 13, 2019Publication date: October 22, 2020Applicants: ArcticZymes AS, Universitetet I Tromsø - Norges Arktiske UniversitetInventors: Terese SOLSTAD, Elisabeth Lill ANDREASSEN, Marit Sjo LORENTZEN, Olav LANES, Morten ELDE, Atle Noralf LARSEN, Yvonne PIOTROWSKI, Nils Peder WILLASSEN
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Patent number: 10787702Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: GrantFiled: August 13, 2019Date of Patent: September 29, 2020Assignees: ARCTICZYMES AS, UNIVERSITETET I TROMSø—NORGES ARKTISKE UNIVERSITETInventors: Terese Solstad, Elisabeth Lill Andreassen, Marit Sjo Lorentzen, Olav Lanes, Morten Elde, Atle Noralf Larsen, Yvonne Piotrowski, Nils Peder Willassen
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Publication number: 20200071753Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: ApplicationFiled: August 13, 2019Publication date: March 5, 2020Applicants: ArcticZymes AS, Universitetet I Tromsø - Norges Arktiske UniversitetInventors: Terese SOLSTAD, Elisabeth Lill ANDREASSEN, Marit Sjo LORENTZEN, Olav LANES, Morten ELDE, Atle Noralf LARSEN, Yvonne PIOTROWSKI, Nils Peder WILLASSEN
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Patent number: 10415082Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: GrantFiled: August 28, 2018Date of Patent: September 17, 2019Assignees: ARCTICZYMES AS, UNIVERSITETET I TROMSø—NORGES ARKTISKE UNIVERSITETInventors: Terese Solstad, Elisabeth Lill Andreassen, Marit Sjo Lorentzen, Olav Lanes, Morten Elde, Atle Noralf Larsen, Yvonne Piotrowski, Nils Peder Willassen
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Publication number: 20180363042Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: ApplicationFiled: August 28, 2018Publication date: December 20, 2018Applicants: ArcticZymes AS, Universitetet I Tromsø - Norges Arktiske UniversitetInventors: Terese Solstad, Elisabeth Lill Andreassen, Marit Sjo Lorentzen, Olav Lanes, Morten Elde, Atle Noralf Larsen, Yvonne Piotrowski, Nils Peder Willassen
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Patent number: 10087483Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCI, pH 8.5 at 25° C., 50 mM KCI and 5 mM MgCI2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: GrantFiled: August 19, 2015Date of Patent: October 2, 2018Assignees: ARCTICZYMES AS, UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITETInventors: Terese Solstad, Elisabeth Lill Andreassen, Marit Sjo Lorentzen, Olav Lanes, Morten Elde, Atle Noralf Larsen, Yvonne Piotrowski, Nils Peder Willassen
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Publication number: 20170233800Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.Type: ApplicationFiled: August 19, 2015Publication date: August 17, 2017Applicants: ArcticZymes AS, Universitetet I Tromsø - Norges Arktiske UniversitetInventors: Terese SOLSTAD, Elisabeth Lill ANDREASSEN, Marit Sjo LORENTZEN, Olav LANES, Morten ELDE, Atle Noralf LARSEN, Yvonne PIOTROWSKI, Nils Peder WILLASSEN
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Patent number: 9133447Abstract: The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.Type: GrantFiled: September 25, 2013Date of Patent: September 15, 2015Assignee: Biotec Pharmacon ASAInventors: Olav Lanes, Morten Elde, Dag Rune Gjellesvik
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Publication number: 20140093938Abstract: The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.Type: ApplicationFiled: September 25, 2013Publication date: April 3, 2014Applicant: BIOTEC PHARMACON ASAInventors: Olav Lanes, Morten Elde, Dag Rune Gjellesvik
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Patent number: 8551753Abstract: The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.Type: GrantFiled: July 21, 2010Date of Patent: October 8, 2013Assignee: Biotec Pharmacon ASAInventors: Olav Lanes, Morten Elde, Dag Rune Gjellesvik
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Publication number: 20110020878Abstract: The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.Type: ApplicationFiled: July 21, 2010Publication date: January 27, 2011Applicant: BIOTEC PHARMACON ASAInventors: Olav Lanes, Morten Elde, Dag Rune Gjellesvik