Abstract: Provided herein are methods and compositions to make guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs from any source nucleic acid. Also provided herein are methods and compositions to use the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs in a variety of applications.

Abstract: Provided are compositions and methods of making a guide nucleic acids (gNAs), methods of using gNAs. and ligation free methods of preparing libraries of nucleic acids for downstream applications such as high-throughput sequencing.

Abstract: The present disclosure provides methods and systems directed to cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or elevated risk of a pregnancy-related state of a pregnant subject may comprise assaying a cell-free biological sample derived from said pregnant subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or elevated risk of the pregnancy-related state.

Abstract: The present invention provides a method for preventing frost damage to one or more flow meters installed in a fluid distribution network containing a fluid, wherein each flow meter is configured to measure a temperature, preferably an air temperature, and to wirelessly transmit the measured temperature, such as via a mobile or a fixed wireless reading system to a processing unit. The method comprises: 1) during a period continuously monitoring (M_TMP) the temperatures measured by the plurality of flow meters over a period of time, 2) performing a data analysis (P_DA) on the temperatures of the plurality of flow meters from the period, 3) based on the data analysis, identifying (I_FDC) one or more ones of the plurality of flow meters as frost damage candidates in a future frost period, and 4) transmitting (T_WS) a warning signal indicative of the frost damage candidates or initiating frost protection measures to protect the frost damage candidates.

Abstract: The present invention provides a method for preventing frost damage to one or more flow meters installed in a fluid distribution network containing a fluid, wherein each flow meter is configured to measure a temperature, preferably an air temperature, and to wirelessly transmit the measured temperature, such as via a mobile or a fixed wireless reading system to a processing unit. The method comprises: 1) during a period continuously monitoring (M_TMP) the temperatures measured by the plurality of flow meters over a period of time, 2) performing a data analysis (P_DA) on the temperatures of the plurality of flow meters from the period, 3) based on the data analysis, identifying (I_FDC) one or more ones of the plurality of flow meters as frost damage candidates in a future frost period, and 4) transmitting (T_WS) a warning signal indicative of the frost damage candidates or initiating frost protection measures to protect the frost damage candidates.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: The present disclosure provides methods and systems directed to cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from said subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Provided herein are compositions and methods for generating libraries for high throughput sequencing without using ligation, and methods of using same.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Methods and systems may perform cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from the subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: The present disclosure provides methods and systems directed to cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from said subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: The present disclosure provides methods and systems directed to cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from said subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Provided are compositions and methods of making a guide nucleic acids (gNAs), methods of using gNAs, and ligation free methods of preparing libraries of nucleic acids for downstream applications such as high-throughput sequencing.

Abstract: The present disclosure provides methods and systems directed to cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from said subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Provided herein are methods and compositions to make guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs from any source nucleic acid. Also provided herein are methods and compositions to use the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs in a variety of applications.

Abstract: The invention relates to the manufacture of a reinforced slab-shaped building element (E) having a length (L), a width (W) and a thickness, said slab-shaped building element (E) comprising an upper concrete plate anchored to a lower concrete plate with a top surface and a bottom surface, said upper concrete plate being cast from relatively higher strength concrete laid out upon said top surface, said lower concrete plate being of a less strong concrete, said lower concrete plate including a base contiguous with a plurality of raised portions integral therewith, said raised portions being spaced apart in the direction of said length (L) and said width (W), said plurality of raised portions defining between them a network of recesses, at least some of said recesses including reinforcing bars (R), said raised portions and said recesses together defining said top surface.