Abstract: Inhibitory bi-specific antibody-like constructs comprising two different single-chain variable fragments (scFv), one targeted against the receptor AXL and one against programmed cell death protein 1 (PD-1) are provided. These bi-specific constructs simultaneously target AXL and PD-1 on cancer and/or immune cells and effectively inhibit tumor growth. Compositions comprising the bi-specific molecules and their use for treatment of cancer, are also disclosed.

Abstract: A polynucleotide is provided. The polynucleotide encoding a chimeric polypeptide comprising an amino acid sequence of an immune checkpoint molecule capable of binding a ligand thereof, the immune checkpoint molecule being translationally fused to another amino acid sequence of a cell signaling module such that upon binding of the immune checkpoint molecule to the ligand, the cell signaling module is activated. Also provided other configurations of the polynucleotide, cells comprising the polynucleotides and methods of using the cells expressing the polynucleotides.

Abstract: Provided herein methods for treating a subject suffering from a disease or a disorder associated with hyperproliferating cells, by irradiating the cells with low-frequency ultrasound at an intensity that is either below the cavitational threshold intensity for the ultrasound frequency, or is characterized by a low-frequency mechanical index below 2.5. The methods may further comprise determining an elasticity value of the cells, and adjusting the ultrasound intensity accordingly.