Patents by Inventor Motohiro Fukuda
Motohiro Fukuda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10836996Abstract: To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.Type: GrantFiled: January 29, 2016Date of Patent: November 17, 2020Assignees: Corning Incorporated, Public University Corporation Yokohama City UniversityInventors: Yoko Itchoda, Go Tazaki, Motohiro Fukuda, Hitoshi Tsuruta, Hideki Taniguchi
-
Patent number: 10513685Abstract: Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 ?m to 500 ?m and a bottom area of 100 ?m2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).Type: GrantFiled: March 23, 2011Date of Patent: December 24, 2019Assignees: CORNING INCORPORATED, PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITYInventors: Yoko Itchoda, Go Tazaki, Masaya Hosoda, Motohiro Fukuda, Hideki Taniguchi, Yun-Wen Zheng, Keisuke Sekine
-
Patent number: 9428721Abstract: Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.Type: GrantFiled: February 5, 2009Date of Patent: August 30, 2016Assignees: Public University Corporation Yokohama City University, KURARAY Co., Ltd.Inventors: Hideki Taniguchi, Yun-Wen Zheng, Go Tazaki, Tomoko Kosaka, Hitoshi Tsuruta, Motohiro Fukuda
-
Publication number: 20160160175Abstract: To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.Type: ApplicationFiled: January 29, 2016Publication date: June 9, 2016Applicants: KURARAY CO., LTD., Public University Corp. Yokohama City UniversityInventors: Yoko ITCHODA, Go Tazaki, Motohiro Fukuda, Hitoshi Tsuruta, Hideki Taniguchi
-
Patent number: 9290735Abstract: To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.Type: GrantFiled: February 5, 2009Date of Patent: March 22, 2016Assignees: National University Corporation Nagoya University, KURARAY Co., Ltd.Inventors: Masahiro Sokabe, Kishio Furuya, Go Tazaki, Hitoshi Tsuruta, Motohiro Fukuda
-
Patent number: 9005970Abstract: [PROBLEMS] Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. [MEANS FOR SOLVING PROBLEMS] A cell culture plate having a plural number of side walls (1) and a plural number of space structures (3) for providing cultured cells which are formed by the side walls (1), wherein the side walls (1) are provided with openings (2) so that the space structures (3) are linked together. By using this cell culture plate, a method of culturing cells under regulation in the extension direction can be provided.Type: GrantFiled: January 11, 2006Date of Patent: April 14, 2015Assignees: Kuraray Co., Ltd., Ryosuke NishioInventors: Ryosuke Nishio, Taiji Nishi, Motohiro Fukuda, Yasuzo Kirita, Seiichi Kanai, Takenori Kitani
-
Patent number: 8980625Abstract: A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.Type: GrantFiled: February 24, 2006Date of Patent: March 17, 2015Assignees: National Food Research Institute, Kuraray Co., Ltd.Inventors: Yuji Kikuchi, Hiroko Kikuchi, Taiji Nishi, Takenori Kitani, Yukihiro Yanagawa, Motohiro Fukuda
-
Publication number: 20140065659Abstract: In a culture method for adipocytes, seeded adipocytes are cultured while being allowed to adhere to a culture bottom surface (14) and walls (12) disposed perpendicularly to the culture bottom surface (14), without suspending the adipocytes in a culture solution, thereby obtaining mature adipocytes in which spherical lipid droplets having increased in size are generated within cells. This method uses a culture chamber (10) in which the walls (12) are formed perpendicular to the culture bottom surface (14). Spherical mature adipocytes containing spherical lipid droplets having increased in size within cells are cultured in a state where a cell or an aggregate of cells is allowed to adhere to two or more locations on the walls (12) and the culture bottom surface (14), thereby obtaining adipocytes having a shape similar to that of adipocytes in vivo.Type: ApplicationFiled: March 16, 2012Publication date: March 6, 2014Applicant: Kuraray Co., Ltd.Inventors: Yoko Itchoda, Masaya Hosoda, Motohiro Fukuda, Masafumi Nagayama
-
Patent number: 8652833Abstract: To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.Type: GrantFiled: April 25, 2007Date of Patent: February 18, 2014Assignee: Kuraray Co., Ltd.Inventors: Yuji Morimoto, Taiji Nishi, Taisuke Kamada, Go Tazaki, Motohiro Fukuda
-
Patent number: 8435782Abstract: An object of the present invention is to provide a cell culture chamber with which the survival rate of cells to be cultured can be increased, and a method of producing the same. A cell culture chamber according to the present invention includes a mass of projections formed of a plurality of microprojections 1 formed on a surface on which cells are cultured. The width or diameter of each of the microprojections 1 is in a range of 20 nm to 3 ?m, and the aspect ratio of each of the microprojections is in a range of 0.2 to 3.0. Thus, there can be provided a cell culture chamber most suitable for the adhesion and differentiation/proliferation of cells to be cultured.Type: GrantFiled: February 19, 2007Date of Patent: May 7, 2013Assignee: Kuraray Co., Ltd.Inventors: Taiji Nishi, Go Tazaki, Motohiro Fukuda, Michio Yazawa, Masayuki Takahashi
-
Patent number: 8415157Abstract: A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber (10)according to the present invention is a cell culture chamber (10)including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.Type: GrantFiled: September 9, 2011Date of Patent: April 9, 2013Assignee: KURARAY Co., Ltd.Inventors: Go Tazaki, Tomoko Kosaka, Motohiro Fukuda
-
Publication number: 20130071932Abstract: Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 ?m to 500 ?m and a bottom area of 100 ?m2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).Type: ApplicationFiled: March 23, 2011Publication date: March 21, 2013Applicants: Public University Corporation Yokohama City University, KURARAY Co., Ltd.Inventors: Yoko Itchoda, Go Tazaki, Masaya Hosoda, Motohiro Fukuda, Hideki Taniguchi, Yun-Wen Zheng, Keisuke Sekine
-
Publication number: 20120199816Abstract: A photoelectric conversion device according to the present invention includes, between a pair of electrodes, an electron donor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting electrode main surfaces being formed at intervals in the stripe-like part in cross section; and an electron acceptor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting the electrode main surfaces being formed at intervals in the stripe-like part in cross section, the photoelectric conversion device further including an active layer in which the plurality of strip-like parts in cross section of the electron donor layer and the plurality of strip-like parts in cross section of the electron acceptor layer are alternately joined.Type: ApplicationFiled: August 2, 2010Publication date: August 9, 2012Applicant: KURARAY CO., LTD.Inventors: Go Tazaki, Motohiro Fukuda, Hiroyuki Ohgi, Akio Fujita
-
Publication number: 20110318829Abstract: To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber (10) according to the present invention is a cell culture chamber (10) including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.Type: ApplicationFiled: September 9, 2011Publication date: December 29, 2011Applicant: KURARAY CO., LTD.Inventors: Go TAZAKI, Tomoko Kosaka, Motohiro Fukuda
-
Publication number: 20110217725Abstract: To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.Type: ApplicationFiled: October 23, 2009Publication date: September 8, 2011Applicants: Kuraray Co., Ltd., Public University Corp. Yokohama City UniversityInventors: Yoko Itchoda, Go Tazaki, Motohiro Fukuda, Hitoshi Tsuruta, Hideki Taniguchi
-
Publication number: 20110207215Abstract: To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells (40) by using a cell culture chamber (20) including a plurality of the microchambers (11), the method including: culturing the living cells by being adhered to the surfaces of a plurality of micro spaces; and after the culturing, pouring the culture medium (50) into the cell culture chamber (20) so as to cover the plurality of microchambers (11), and storing the living cells. The living cells are adhered to a cell culture chamber of an appropriate size and cultured to form a three-dimensional structure. This enables maintaining the three-dimensional structure of the living cells and storage of the living cells while maintaining the functions thereof.Type: ApplicationFiled: October 23, 2009Publication date: August 25, 2011Applicants: KURARAY CO., LTD., PUBLIC UNIVERSITY CORP. YOKOHAMA CITY UNIVERSITYInventors: Yoko Itchoda, Go Tazaki, Motohiro Fukuda, Hitoshi Tsuruta, Hideki Taniguchi
-
Patent number: 7972543Abstract: An object of the present invention is to provide a process for producing microspheres that are solid microparticles or liquid microparticles for use as an emulsion employed in the food industry, production of medicine and cosmetic etc. or an emulsion for DDS (drug delivery system). The object is attained by a process for producing a microsphere including: separating a disperse phase from a continuous phase by a substrate 1 having a through-hole 7; and extruding the disperse phase into the continuous phase through the through-hole 7, in which the substrate having the through-hole 7 with a width of 0.5 to 500 ?m, a depth of 10 ?m to 6000 ?m and a ratio of the width to the depth of the through-hole 7 of 1 to 1/30 is a metal substrate.Type: GrantFiled: October 11, 2005Date of Patent: July 5, 2011Assignees: National Agriculture and Food Research Organization, Kuraray Co., Ltd.Inventors: Mitsutoshi Nakajima, Taiji Nishi, Seiichi Kanai, Takenori Kitani, Motohiro Fukuda
-
Publication number: 20110045500Abstract: Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.Type: ApplicationFiled: February 5, 2009Publication date: February 24, 2011Applicants: PUBLIC UNIVERSITY CORP. YOKOHAMA CITY UNIVERSITY, KURARAY CO., LTD.Inventors: Hideki Taniguchi, Yun-Wen Zheng, Go Tazaki, Tomoko Kosaka, Hitoshi Tsuruta, Motohiro Fukuda
-
Publication number: 20100331216Abstract: To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.Type: ApplicationFiled: February 5, 2009Publication date: December 30, 2010Applicants: National University Corporation Nagoya University, Kuraray Co., Ltd.Inventors: Masahiro Sokabe, Kishio Furuya, Go Tazaki, Hitoshi Tsuruta, Motohiro Fukuda
-
Publication number: 20100190253Abstract: To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber (10) according to the present invention is a cell culture chamber (10) including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.Type: ApplicationFiled: June 16, 2008Publication date: July 29, 2010Applicant: KURARAY CO., LTD.Inventors: Go Tazaki, Tomoko Kosaka, Motohiro Fukuda