Abstract: The present invention relates to a method of treating a liquid composition containing lacto-N-biose, the method including: preparing a liquid composition containing lacto-N-biose having a pH at 25° C. of not less than 2.0 and not more than 5.5, and heating the liquid composition at a temperature of 65° C. or higher. The present invention can provide a treatment method that enables thermal decomposition of lacto-N-biose to be suppressed when a liquid composition containing lacto-N-biose is heated at a temperature of 65° C. or higher, a liquid preparation containing lacto-N-biose that is treated using the treatment method, a dried product produced from such a liquid preparation containing lacto-N-biose, and a method of producing a product containing lacto-N-biose.

Abstract: The present invention relates to a method of treating a liquid composition containing lacto-N-biose, the method including: preparing a liquid composition containing lacto-N-biose having a pH at 25° C. of not less than 2.0 and not more than 5.5, and heating the liquid composition at a temperature of 65° C. or higher. The present invention can provide a treatment method that enables thermal decomposition of lacto-N-biose to be suppressed when a liquid composition containing lacto-N-biose is heated at a temperature of 65° C. or higher, a liquid preparation containing lacto-N-biose that is treated using the treatment method, a dried product produced from such a liquid preparation containing lacto-N-biose, and a method of producing a product containing lacto-N-biose.

Abstract: A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided. The method for producing lacto-N-biose I or galacto-N-biose, characterized in that the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give ?-glucose-1-phosphate; and (ii) a combination of an enzyme that converts ?-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to galactose-1-phosphate with their cofactors, and/or a combination of an enzyme (UDP-Gly synthase) that converts ?-glucose-1-phosphate and UDP-galactose to UDP-glucose and ?-galactose-1-phosphate, respectively, with its cofactor(s) to act in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), and UDP-glucose-4-epimerase (EC 5.1.3.2).

Abstract: A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided. The method for producing lacto-N-biose I or galacto-N-biose, characterized in that the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give a-glucose-1-phosphate; and (ii) a combination of an enzyme that converts ?-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to galactose-1-phosphate with their cofactors, and/or a combination of an enzyme (UDP-Gly synthase) that converts ?-glucose-1-phosphate and UDP-galactose to UDP-glucose and ?-galactose-1-phosphate, respectively, with its cofactor(s) to act in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), and UDP-glucose-4-epimerase (EC 5.1.3.2).

Abstract: A process for extraction of a carotenoid compound from bacterial cells containing the carotenoid compound comprising the step of bringing the bacterial cells into contact with supercritical fluid so as to extract the carotenoid compound from the cells. The process provides carotenoid compounds which can be safely used as feed additives and food additives.

Abstract: A method for preparing cellobiose from sucrose in a high yield at low cost without difficulty by using three enzymes of sucrose phosphorylase, glucose isomerase and cellobiose phosphorylase in the presence of orthophosphate. The additional method of the invention comprises 4 steps: to treat sucrose with sucrose phosphorylase in the presence of orthophosphate to produce fructose and glucose-1-phosphate; to treat the fructose obtained in the preceding step with glucose isomerase to produce glucose; to treat the glucose obtained in the preceding step and the glucose-1-phosphate obtained in the first step with cellobiose phosphorylase to produce cellobiose and orthophosphate; and to recover at least a part of cellobiose from the reaction mixture in the preceding step and to recycle at least a part of the remaining reaction mixture containing orthophosphate to the first step.