Abstract: Chemiluminescent substances are prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound. The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water-soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

Abstract: The present invention provides a method and apparatus for measuring the membrane potential of red blood cells using electrophoretic analysis that analyzes red blood cells whose membrane potentials are reversed due to red blood cell agglutination.

Abstract: An injector has a sample chamber for retaining a small amount of sample, wherein a position of the sample chamber is controlled by transmitting light through the sample chamber and detecting the transmitted light so as to recognize the position of the sample chamber. It is possible to reduce detection error compared to the conventional injectors that indirectly control the position of the sample chamber by transmitting light through the light passage which is adjacent to the sample chamber. A method for injecting a sample comprising the steps of: providing an injector which has a sample chamber for retaining a sample with an open month; transmitting light through the sample chamber; detecting the transmitted light so as to recognize a position of the sample chamber; and controlling the position of the sample chamber by using the detection result to connect the open month of the sample chamber to an open month of a sample receiving member in order to inject the sample into the sample receiving member.

Abstract: The present invention provides an organic monolith reactor and the preparation method thereof, wherein the reactor is possible to manufacture even into a small size and even at room temperature, and the manufacturing process is simple and relatively short period of time is required. In an organic monolith reactor according to the present invention, a horseradish peroxidase is embedded onto a microscopic area with a photopolymerized polymer. The method of preparing an organic monolith reactor according to the present invention comprises (a) dispersing a horseradish peroxidase in a mixed solution of a photopolymerizable monomer and a photopolymerizing agent, (b) introducing the dispersed solution into a microscopic area, and (c) performing a photo-irradiation onto the microscopic area at room temperature.

Abstract: Disclosed herein are novel chemiluminescent substances prepared by freezing or lyophilizing a solution of a peroxidized nitrogen-containing five-membered cyclic compound. The chemiluminescent substances have a sensitivity comparable to that of luminol chemiluminescence, are highly water soluble, have no incorporation of impurities, are not degraded during storage, have high reproducibility in measurement results, and are very suitable for use in the detection and quantification of various substances in many applications, e.g., clinical applications.

Abstract: A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

Abstract: Disclosed herein are a chemiluminescence chip, which is used to detect and quantify various kinds of materials in the case of performing the enzyme labeling of a material to be measured and performing the chemiluminescence measurement of the activity of the labeled enzyme, and a chemiluminescence detecting apparatus using the same. The chemiluminescence chip is constructed such that a filling unit, in which an immobilized enzyme is filled, is mounted in a space having an inlet port, through which a material to be measured is introduced, and an outlet port, through which the material to be measured is discharged, on a supporting structure.

Abstract: An injector has a sample chamber for retaining a small amount of sample, wherein a position of the sample chamber is controlled by transmitting light through the sample chamber and detecting the transmitted light so as to recognize the position of the sample chamber. It is possible to reduce detection error compared to the conventional injectors that indirectly control the position of the sample chamber by transmitting light through the light passage which is adjacent to the sample chamber. A method for injecting a sample comprising the steps of: providing an injector which has a sample chamber for retaining a sample with an open month; transmitting light through the sample chamber; detecting the transmitted light so as to recognize a position of the sample chamber; and controlling the position of the sample chamber by using the detection result to connect the open month of the sample chamber to an open month of a sample receiving member in order to inject the sample into the sample receiving member.

Abstract: A method for determining the distribution of glycation of erythrocytic membranes includes steps of bonding magnetic beads having a boric acid group with sugars on the surfaces of erythrocytic membranes, and moving the erythrocytes bonded with the boric acid-magnetic beads under an influence of an electromagnetic force. A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline.

Abstract: An injector has a sample chamber for retaining a small amount of sample, wherein a position of the sample chamber is controlled by transmitting light through the sample chamber and detecting the transmitted light so as to recognize the position of the sample chamber, and it is possible to reduce detection error compared to the conventional injectors that indirectly control the position of the sample chamber by transmitting light through the light passage which is adjacent to the sample chamber. A method for injecting a sample comprising the steps of: providing an injector which has a sample chamber for retaining a sample with an open month; transmitting light through the sample chamber; detecting the transmitted light so as to recognize a position of the sample chamber; and controlling the position of the sample chamber by using the detection result to connect the open month of the sample chamber to an open month of a sample receiving member in order to inject the sample into the sample receiving member.

Abstract: An injector has a sample chamber for retaining a small amount of sample, wherein a position of the sample chamber is controlled by transmitting light through the sample chamber and detecting the transmitted light so as to recognize the position of the sample chamber, and it is possible to reduce detection error compared to the conventional injectors that indirectly control the position of the sample chamber by transmitting light through the light passage which is adjacent to the sample chamber. A method for injecting a sample comprising the steps of: providing an injector which has a sample chamber for retaining a sample with an open month; transmitting light through the sample chamber; detecting the transmitted light so as to recognize a position of the sample chamber; and controlling the position of the sample chamber by using the detection result to connect the open month of the sample chamber to an open month of a sample receiving member in order to inject the sample into the sample receiving member.

Abstract: A cell for sample detection in chromatography includes a material fixed therein, whose translucency is changed or increased by an interaction with samples. A method for detecting samples in chromatography includes the steps of providing a light-emitting means and a light-receiving means, providing the cell between the light-emitting means and the light-receiving means, making the samples flow through the cell and detecting the components of the samples by utilizing the phenomenon that the translucency of the material fixed in the cell is changed or increased by the interaction with the samples. A sample detector for chromatography includes a light-emitting means, a light-receiving means, and the cell placed between the light-emitting means and the light-receiving means.

Abstract: A method for determining the distribution of glycation of erythrocytic membranes includes steps of bonding magnetic beads having a boric acid group with sugars on the surfaces of erythrocytic membranes, and moving the erythrocytes bonded with the boric acid-magnetic beads under an influence of an electromagnetic force. A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline.

Abstract: The liquid pump has a pressure vessel with end-openings for hermetically holding liquid therein. The liquid flows from one of the end-openings to the other end-opening by utilizing a difference in a coefficient of thermal expansion of the pressure vessel and the liquid caused by heating or cooling the pressure vessel and the liquid together. The pressure vessel is a tube having an inner diameter of about 0.5 mm or less and a length of about 10 m or more.

Abstract: A change in physical properties of a fluid within an open tube which is caused by heating an arbitrary portion in the open tube by heating means is detected by using a detector disposed with a predetermined spacing from the arbitrary portion, thereby measuring the flow in the open tube. The present invention provides a method for measuring flow within an open tube, in which a mechanical error and contamination of a passage do not occur, as a method for measuring flow within an open tube having a very small diameter provided for the separation analysis field and chemosynthesis field.

Abstract: A material is provided for capturing a chemical substance comprising fibers which carry metal alkoxide particles on the surfaces thereof. The metal alkoxide particles are made from an oligosilica, an oligozirconia, an oligotitiania, or the like. The fibers may include inorganic fibers, regenerated fibers and/or synthetic fibers. The material for capturing a chemical substance is produced by a method comprising steps of subjecting the fibers to a surface treatment for facilitating adhesion of the metal alkoxide particles to the surfaces of the fibers, forming the metal alkoxide particles on the surfaces of the fibers, and fixing the metal alkoxide particles to the surfaces of the fibers. A chemical substance-capturing tube, capable of capturing a trace of a chemical substance in a liquid or a gas, can be produced by placing the fibers, i.e., the material for capturing a chemical substance, in a hollow tube.

Abstract: In an open capillary column, a stationary phase is formed on the inner wall of a capillary tube. This stationary phase comprises a number of pores on the surface of the capillary tube, and each of pore has an expanded hollow in the stationary phase. A manufacturing method of an open capillary column comprises the steps of giving alkaline treatment to an inner wall of an open capillary tube, applying one of oligo silica, oligo zirconia and oligo titania on the inner wall, filling the open capillary tube with alkaline solution to form one of a silica layer, a zirconia layer and titania layer, discharging the alkaline solution and heat-treating the capillary tube to form a base of a stationary phase. The open capillary column of the present invention comprises a stationary phase having a very large surface area. It is useful for a separation analysis field such as liquid chromatography and electrochromatography.

Abstract: A material is provided for capturing a chemical substance comprising fibers which carry metal alkoxide particles on the surfaces thereof. The metal alkoxide particles are made from an oligosilica, an oligozirconia, an oligotitiania, or the like. The fibers may include inorganic fibers, regenerated fibers and/or synthetic fibers. The material for capturing a chemical substance is produced by a method comprising steps of subjecting the fibers to a surface treatment for facilitating adhesion of the metal alkoxide particles to the surfaces of the fibers, forming the metal alkoxide particles on the surfaces of the fibers, and fixing the metal alkoxide particles to the surfaces of the fibers. A chemical substance-capturing tube, capable of capturing a trace of a chemical substance in a liquid or a gas, can be produced by placing the fibers, i.e., the material for capturing a chemical substance, in a hollow tube.

Abstract: In an open capillary column, a stationary phase is formed on the inner wall of a capillary tube. This stationary phase comprises a number of pores on the surface of the capillary tube, and each of pore has an expanded hollow in the stationary phase. A manufacturing method of an open capillary column comprises the steps of giving alkaline treatment to an inner wall of an open capillary tube, applying one of oligo silica, oligo zirconia and oligo titania on the inner wall, filling the open capillary tube with alkaline solution to form one of a silica layer, a zirconia layer and titania layer, discharging the alkaline solution and heat-treating the capillary tube to form a base of a stationary phase. The open capillary column of the present invention comprises a stationary phase having a very large surface area. It is useful for a separation analysis field such as liquid chromatography and electrochromatography.

Abstract: A change in physical properties of a fluid within an open tube which is caused by heating an arbitrary portion in the open tube by heating means is detected by using a detector disposed with a predetermined spacing from the arbitrary portion, thereby measuring the flow in the open tube. The present invention provides a method for measuring flow within an open tube, in which a mechanical error and contamination of a passage do not occur, as a method for measuring flow within an open tube having a very small diameter provided for the separation analysis field and chemosynthesis field.