Abstract: The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an mRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein.

Abstract: The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an mRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein.

Abstract: A sample tube of a sampling valve is moved up and down by a cylinder. When the sample tube is moved downward, a sample inlet opposes a stored sample, and the sample can be extracted. When the sample tube is moved upward, the sample inlet opposes a cleaning liquid path. Thus, cleaning liquid is fed into the cleaning liquid path from a cleaning liquid supply port of a cleaning pipe. The cleaning liquid flows within the sample tube from the sample inlet, and flows out from a sample discharge port. Thus, an entire interior of the sample tube can be cleaned. In a sample extracting device using the sampling valve, a state in which no air or impurities are mixed in the sample can always be maintained, such that stable extraction can be carried out.

Abstract: A sample tube of a sampling valve is moved up and down by a cylinder. When the sample tube is moved downward, a sample inlet opposes a stored sample, and the sample can be extracted. When the sample tube is moved upward, the sample inlet opposes a cleaning liquid path. Thus, cleaning liquid is fed into the cleaning liquid path from a cleaning liquid supply port of a cleaning pipe. The cleaning liquid flows within the sample tube from the sample inlet, and flows out from a sample discharge port. Thus, an entire interior of the sample tube can be cleaned. In a sample extracting device using the sampling valve, a state in which no air or impurities are mixed in the sample can always be maintained, such that stable extraction can be carried out.

Abstract: A method is proposed for the production of high-strength low-expansion cast iron enabled to acquire improved strength, hardness, and cutting workability while retaining the property of low expansion intact. The product is a low-expansion cast iron having a high nickel content and exhibiting a coefficient of thermal expansion of not more than 8.times.10.sup.-6 /.degree. C. at temperatures in the range of from room temperature to 100.degree. C. By causing a carbide to be finely precipitated in an area ratio in the range of from 0.3% to 20% in the metal structure of the cast iron and lowering the C content in the cast iron, there is produced a high-strength low-expansion cast iron. The deposition of the carbide mentioned above is accomplished by incorporating in the material for cast iron at least one element selected from the group consisting of the transition metal elements of IVa, Va, and VIa Groups in the Periodic Table of the Elements.

Abstract: A seal diaphragm structure for a pressure measuring device includes a seal diaphragm, an insulating film, and an p-type semiconductor film. The seal diaphragm has a first surface opposing a target fluid and a second surface contacting a pressure transfer sealed liquid sealed in the pressure measuring device. The insulating film is formed on the first surface of the seal diaphragm. The p-type semiconductor film is formed on the insulating film.

Abstract: A cast iron having an austenitic matrix and consisting essentially of from 1% up to 3.5% carbon, up to 1.5% silicon, from 32% to 39.5% nickel, from 1% to less than 4% cobalt, up to 41% of the combined total of nickel plus cobalt and the balance substantially all iron provides a low expansion coefficient, good castability, good cutting properties and good damping capacity.

Abstract: This invention is directed to a cast iron having a graphite structure in an austenitic iron matrix and consisting essentially of, in weight %, at least about 1.0% and not more than about 3.5% of carbon, not more than about 1.0% of silicon, at least about 29% and not more than about 34% of nickel, at least about 4% and not more than about 8% of cobalt and the balance substantially all iron.

Abstract: A lapping tool with a flatness in the range below 20 .mu.m which is made of at least cast iron having an austenitic matrix and consisting essentially of from 1% up to 3.5% carbon, up to 1.5% silicon, from 32% to 39.5% nickel, from 1% to less than 4% cobalt, up to 41% of the combined total of nickel plus cobalt and the balance substantially all iron providing a low expansion coefficient, good castability, good cutting properties and good damping capacity. (By % is meant % by weight).

Abstract: A fluid circuit device provided with a fluid circuit formed by a base member and a thin sheet integrally electrocast on said base member and methods for producing the fluid circuit device with parallel and non-parallel fluid circuits.

Abstract: An exhaust-gas purifier comprising a monolithic catalyst which is disposed in an exhaust passage of an engine proper provided with a plurality of cylinders, and which has a plurality of paths running in the direction of the exhaust-gas stream, wherein the exhaust passage is divided into a plurality of passageways opening close to the upstream end of the monolithic catalyst, the passageways being independent from each other or combined with proper others so as to form compound passageways. The part of the independent exhaust passageways corresponding to each cylinder or compound passageways is extended to the downstream end of the monolithic catalyst, substantially separated from each other, thus lowering the interference of each cylinder due to fluctuation of the exhaust pressure exerting adverse effects on each other.

Abstract: A pneumatic control apparatus is disclosed for an engine exhaust emission control device which includes a passage 101 for applying a negative intake passage pressure to a chamber of a differential pressure responsive unit 10 an atmospheric venting vacuum control valve 30 is provided for selectively venting the passage 101. The negative pressure chamber 37 of the valve 30 is controlled by an orifice unit 40 which supplies atmospheric pressure and/or negative pressures from spaced positions in the intake passage proximate the throttle valve 2 via flow restrictors 43, 44, whereby suitable response delays are provided. A further restrictor and check valve connected in parallel may also be employed to alter the response time. The proper selection of the restrictor flow resistances and the biasing spring strengths in the various control valves enables a wide range of operational control.

Abstract: An engine has separate cylinders to which rich and lean mixtures are respectively supplied. A common carburetor that is set to make a rich air-fuel mixture is used for feeding both rich and lean mixtures. An air-supply passage is connected to an intake manifold that communicates the carburetor with the cylinder to which lean mixture is fed. The air introduced from the air-supply passage is mixed with the rich mixture to produce the desired lean mixture. A valve unit is installed in the air-supply passage, so that the rich mixture is fed to all the cylinders during high-load operation by closing the valve.

Abstract: A thermal reactor comprising a shell, an outer core in the shell, an inner core supported by the outer core, an inlet pipe being fixed to the shell and extending through the outer and inner cores, and an outlet pipe. A convex portion on the periphery of the outer core is slidably supported in a concave portion provided on the shell and one end of the inner core is slidably supported on the periphery of the outer core. The outlet pipe is fixed to the shell and the outer core. Therefore, relative movements caused by the thermal deformations are permitted between the shell and the outer and inner cores, while generations of noise and vibration of each member are prevented.

Abstract: The thermal reactor comprising a shell, a core in the shell, an inlet pipe being fixed at one end to the shell and extending sealingly through the core, and an outlet pipe. A convex portion on the periphery of the core is slidably supported in a concave portion formed in the shell and the outlet pipe is fixed thereto and to the core, thereby permitting relative movements therebetween due to thermal deformation and preventing generation of noise and vibration of each member.