Abstract: The invention discloses a method for improving rice yield and a protein used thereof. The invention provides a method for cultivating the target rice, comprising the following steps of inhibiting the activity of RAY1 protein in original rice to obtain target rice; compared with the original rice, the target rice shows higher yield and/or larger grain size and/or stronger resistance to rice blast and/or higher plant height and/or longer stem internode length; the RAY1 protein is a protein composed of an amino acid sequence shown as SEQ ID No. 1 in a sequence list. The invention uses CRISPR/Cas9 technology to realize site-directed editing rice RAY1 gene, through knocking out rice RAY1 gene by frameshift mutation, the protein RAY1 is inactivated, and a new generation of rice germplasm with significantly improved yield is obtained.

Abstract: The present invention discloses a method for preparing a rice photosensitive male sterile material and related genes thereof. The method for preparing photosensitive male sterile rice of the present invention includes: reducing the abundance of protein RMS1 in the target rice, reducing the activity of the protein RMS1 in the target rice or reducing the content of the protein RMS1 in the target rice to obtain the photosensitive male sterile rice. The protein RMS1 is the following A1) or A2): A1), the amino acid sequence of which is as shown in SEQ ID No. 1 in the sequence listing; A2), a homologous protein having more than 98% identity with A1) and is derived from rice. The present invention obtains a rice photosensitive male sterile material by controlling the RMS1 gene of rice and its encoded protein, and achieves the fertility of rice under different light conditions.

Abstract: The invention discloses a method for improving rice yield and a protein used thereof. The invention provides a method for cultivating the target rice, comprising the following steps of inhibiting the activity of RAY1 protein in original rice to obtain target rice; compared with the original rice, the target rice shows higher yield and/or larger grain size and/or stronger resistance to rice blast and/or higher plant height and/or longer stem internode length; the RAY1 protein is a protein composed of an amino acid sequence shown as SEQ ID No. 1 in a sequence list. The invention uses CRISPR/Cas9 technology to realize site-directed editing rice RAY1 gene, through knocking out rice RAY1 gene by frameshift mutation, the protein RAY1 is inactivated, and a new generation of rice germplasm with significantly improved yield is obtained.