Abstract: A method includes: obtaining, by a computer, subject response data including audio data, video data, or audio-video data of a subject's response to one or more stimuli presented to the subject over a defined time period; extracting at least one subject descriptor from the subject response data as a function of time, the at least one subject descriptor including data characterizing involuntary or voluntary action of the subject in response to the one or more stimuli; deriving, from the at least one subject descriptor, a first biomarker characterizing a behavior of the subject in response to the one or more stimuli, in which a value of the first biomarker is indicative of a severity of a disease in the subject; and outputting a disease severity level for the disease as a function of the value of the first biomarker.

Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol:cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: A computer-implemented method includes obtaining a video of a subject, the video including a plurality of frames; generating, based on the plurality of frames, a plurality of optical flows; and encoding the plurality of optical flows using an autoencoder to obtain a movement-based biomarker value of the subject.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol: cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

Abstract: A computer-implemented method includes obtaining a video of a subject, the video including a plurality of frames; generating, based on the plurality of frames, a plurality of optical flows; and encoding the plurality of optical flows using an autoencoder to obtain a movement-based biomarker value of the subject.

Abstract: A method includes: obtaining, by a computer, subject response data including audio data, video data, or audio-video data of a subject's response to one or more stimuli presented to the subject over a defined time period; extracting at least one subject descriptor from the subject response data as a function of time, the at least one subject descriptor including data characterizing involuntary or voluntary action of the subject in response to the one or more stimuli; deriving, from the at least one subject descriptor, a first biomarker characterizing a behavior of the subject in response to the one or more stimuli, in which a value of the first biomarker is indicative of a severity of a disease in the subject; and outputting a disease severity level for the disease as a function of the value of the first biomarker.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: In some embodiments, an app provides a geolocation-limited marketplace limited to a pre-defined period of time. The geolocation-limited marketplace is accessible only by the users in a predefined geographical area. Typically, the predefined geographical area is limited to an area small enough for a user to walk from one end to the other in a short period of time. The pre-defined period of time is of some limited length of time that may occur at a pre-defined interval. In some instances, the period of time is one hour.

Abstract: A method of cryopreserving sperm cells which may include cooling the quantity of sperm cells to a holding temperature, maintaining the sperm cells substantially at the holding temperature for a period, and supercooling the sperm cells by cooling the sperm cells at a first cooling rate to a temperature that approaches a critical temperature zone at which ice crystal formation and changes in osmotic pressure damage sperm cells and then cooling the sperm at a second cooling rate faster than said first cooling rate through said critical temperature zone.

Abstract: A method of processing animal sperm cells that includes: collecting sperm cells from a male animal; sorting the sperm cells to obtain a quantity of sperm cells having a desired characteristic, the quantity of sorted sperm cells being contained in a first volume of fluid having a first concentration of sperm cells; and subjecting the sperm cells to concentration steps in which the concentration of the sperm cells is increased to a second concentration greater than the first concentration.

Abstract: Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

Abstract: Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40° C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.

Abstract: Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

Abstract: Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40° C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.