Abstract: Chronic wounds are medical care concern and severe clinical challenge worldwide. A nanofiber based new wound dressing scaffold and a method of developing thereof for treating diabetic wounds is described here. More specifically, the present invention relates to the preparation of biocompatible Polyacrylonitrile (PAN) nanofiber scaffold comprising of Quercetin, Zinc oxide and polyacrylonitrile by electrospinning. The nanofibers formed has the average diameter of approximately 160 nm and show excellent antibacterial and wound healing potentials.