Abstract: Method and system for detecting a fungal gene and kit for use with same. According to one embodiment, a nucleotide capture probe is coupled to a surface, such as a well of a 96-well plate, using a biotin-streptavidin interaction. The capture probe is preferably specific for a portion of a target sequence of a fungal gene of interest. Upon capture, additional portions of the target sequence of the fungal gene of interest are tagged with nucleotide labeling probes. An enzyme label, which is preferably a poly-horseradish peroxidase conjugate, is then attached to each of the labeling probes, for example, by a biotin-streptavidin interaction. The enzyme label catalyzes the oxidation of a substrate, such as 3,3?,5,5?-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The oxidized substrate may then be detected photonically, by visually detecting a colorimetric change or by absorbance readings, and/or detected electrochemically in the presence of an acid.

Abstract: Method and system for detecting drug and/or drug metabolites in a liquid sample, such as a wastewater sample. According to one embodiment, the method involves providing a device that includes a graphene field effect transistor and a first aptamer coupled to the graphene field effect transistor in a first well, the first aptamer being selective for a first drug or drug metabolite. Next, a liquid sample is introduced to the first aptamer of the device. Next, a sweeping liquid gate voltage is applied to the device to obtain a resistance versus liquid gate voltage plot for the device. Next, the Dirac voltage shift, if any, in the liquid gate voltage plot for the device is used to determine the presence and/or quantity of the drug or drug metabolite. Additional aptamers selective for different drugs or drug metabolites of interest may also be included in other wells of the device.