Abstract: A HIV filtration machine and method of filtration using the machine and method of detecting HIV, the said machine comprising an acrylic cylindrical tube divided into three portions i.e. needle portion (2), barrel portion (10), and tray (16), characterized in that the needle portion consists of three needle points (3, 4, 5), the said needle points are detachably connected with three blood carrying tubes (27, 28, 29) serially fixed into barrel portion, a valve (12) is provided at the end point of said blood carrying tubes, a piston (9) is detachably fixed above the plunger (22) and connected with a spring for creating movement of the plunger towards the tray, the said tray is one inch or less diameter in round shape and connected with steel ring of filter (14) and is connected with chip and micro camera, for data transmission to the remote computer or mobile device, the said filter having less than 1 micron to more than 8.5 micron.