Abstract: Provided are methods of generating a metabolically mature human cell selected from the group consisting of a cardiomyocyte, a pancreatic beta cell, and a neuronal cell, using an effective concentration of a conjugated fatty acid and optionally a nonconjugated fatty acid selected from the group consisting of: a monounsaturated omega-9 fatty acid, palmitic acid, linoleic acid (LA) and a short chain fatty acid. Also provided are isolated populations of metabolically mature human cells, and methods and kits using same for selecting a compound for toxicity to the cells.

Abstract: The present disclosure provides, in part, a cell culture medium comprising a serum-free medium and one or more fibroblast growth factor (FGF) activators, a kit comprising the cell culture medium and instructions for use, methods of growing cells in vitro and of producing a cultured meat using the cell culture medium, and a cultured meat so produced.

Abstract: Methods of generating organoids on multi-well plates are provided by depositing a polymeric solution comprising cells under conditions which result in a homogenous population of organoids, which can be used for high throughput analysis for drug screening and for determining treatment regimens of a drug.

Abstract: A culture medium comprising a WNT inhibitor, a SRC inhibitor and a protein kinase C (PKC) inhibitor is disclosed. The medium is devoid of an amount of GSK3beta inhibitor that increases beta-catenin translocation to the nucleus of a pluripotent stem cell being cultured in the culture medium. Uses thereof are also disclosed.