Abstract: A method for preparing phosphatidylinositol from a vegetable matter comprises homogenization thereof in a water-salt solution with a molar concentration of from 0.005 to 1 M at a pH within the range of from 5.0 to 11.0. The homogenizate is centrifugated at a gravity acceleration value of 3,000-30,000 g or filtered on a filter with a pore size of 3-50 .mu.m to separate the cell-free water-salt phase containing particles of the protein-phosphatidylinositol complex which are separated by means of alkaline or neutral proteases or by way of gel-chromatography or ultrafiltration. From the separated particles of the protein-phosphatidylinositol complex the product is extracted with methanol or with distilled water.