Abstract: The present application relates to a method for preparing a pegylated collagen-like protein and the use thereof. The method includes reacting a collagen-like protein and a polyethylene glycol derivative at a molar ratio of the collagen-like protein to the polyethylene glycol derivative of 1 to 16:1 under the conditions of a pH of 6.0-8.0 and a temperature of 2-8° C. to prepare a pegylated collagen-like protein. In some embodiments, the reacting includes a reaction solvent of the collagen-like protein, and the polyethylene glycol derivative is water or a dilute hydrochloric acid solution. In some instances, the method may additionally include a purification step. The purification step may intercept substances with a molecular weight greater than or equal to 30,000 Da in the reaction product by filtration to obtain the PEGylated collagen-like protein.