Abstract: Disclosed herein is a method for binding enzymes comprising reacting either a synthetic or a natural polymer containing carboxyl and amino groups with an excess of a urethane prepolymer followed by coupling of an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood.

Abstract: Disclosed herein is a method for binding enzymes comprising reacting an amino acid and/or a protein with an excess of a urethane prepolymer, curing the resulting product and coupling an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood serum.

Abstract: Support matrices for immobilized enzymes comprise a combined organic-inorganic support consisting of a porous inorganic support containing an organic polymeric material which possesses functionalized pendent groups, said polymeric material being entrapped and also adsorbed in part in the pores of the inorganic porous support. The organic polymeric component of the matrix constitutes a copolymer prepared in situ from a first polyfunctional monomer, a low molecular weight polymer, a low molecular weight polymer hydrolysate, or a preformed polymer of natural or synthetic origin which is reacted with an excess of a second bifunctional reactive monomer.